CBP/KAT3A Antibody

Product Discontinued

CBP/KAT3A Antibody Summary

• Immunogen: Synthetic peptide, corresponding to amino acids 2395-2440 of Human CBP.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CREBBP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000
• Application Notes: Western blot (WB) analysis of CREBBP (V2427) pAb in extracts from HeLa cell treted with Insulin 0.01U/ml 15'.
  Immunohistochemistry (IHC) analyzes of CREBBP (V2427) pAb in paraffin-embedded human breast carcinoma tissue.
• Theoretical MW: 265 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2)
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CBP/KAT3A Antibody

• CBP
• CBPRTS
• CREB binding protein
• CREB-binding protein
• CREBBP
• EC 2.3.1.48
• KAT3A
• RSTS
• RTS
• Rubinstein-Taybi syndrome

Background

Cyclic AMP-regulated gene expression frequently involves a DNA element designated the cAMP-regulated enhancer (CRE). Many transcription factors bind to this element, including the protein CREB, which is activated as a result of phosphorylation by protein kinase A. It has been shown that protein kinase A-mediated CREB phosphorylation results in its binding to a nuclear protein designated CBP (for CREB-binding protein). These findings suggest that CBP has many of the properties expected of a CREB co-activator. Another high molecular weight transcriptional adapter protein, designated p300, is characterized by three cysteine- and histidine-rich regions, of which the most carboxy-terminal region specifically binds the adenovirus E1A protein. p300 molecules lacking an intact E1A binding site bypass E1A repression, even in the presence of high concentrations of E1A. Sequence analysis of CBP and p300 has revealed substantial homology, arguing that these proteins are members of a conserved family of co-activators.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CBP/KAT3A Antibody (NB100-91721)(1)

Publication using NB100-91721

• Huang H, Zhang D, Weng Y et al. The regulatory enzymes and protein substrates for the lysine beta-hydroxybutyrylation pathway Science advances 2021-02-01 [PMID: 33627428] (WB, Human)

Bioinformatics

• Gene Symbol: CREBBP
• Entrez:
  • 1387(Human)
• Uniprot:
  • Q92793()