Cathepsin H Antibody (175909) [Alexa Fluor® 405]

• Applications: WB
• Clone: 175909
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 405

Cathepsin H Antibody (175909) [Alexa Fluor® 405] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Cathepsin H
  Glu22-Val333
  Accession # Q3UCD6
• Specificity: Detects mouse Cathepsin H in direct ELISAs and Western blots. It recognizes both the pro and active forms of recombinant mouse (rm) Cathepsin H. In Western blots, no cross-reactivity with rmCathepsin A, D, E, X, and Z or recombinant hu
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Cathepsin H is a lysosomal cysteine protease of the papain family (1). It is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑95), a mini chain (residues 96‑103), a heavy chain (residues 114‑290) and a light chain (residues 291‑333) (2, 3). A truncated form with a 12 amino acid deletion in the signal peptide region is secreted (4). Cathepsin H is the only known mono-aminopeptidase in the papain family (5). Cathepsin H expression is significantly increased in prostate tumors, sera of asthmatic patients, and mucosa of colorectal cancer patients (4, 6, 7).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.