Cathepsin H Antibody (175909) [Alexa Fluor™ Plus 594]

• Reactivity: Mu
• Clone: 175909
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 594

Cathepsin H Antibody (175909) [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects mouse Cathepsin H in direct ELISAs and Western blots. It recognizes both the pro and active forms of recombinant mouse (rm) Cathepsin H. In Western blots, no cross-reactivity with rmCathepsin A, D, E, X, and Z or recombinant human Cathepsin B, C, L, or S is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cathepsin H Antibody (175909) [Alexa Fluor™ Plus 594]

• ACC-4
• ACC-5
• aleurain
• cathepsin B3
• cathepsin BA
• Cathepsin H
• CPSB
• CTSH
• DKFZp686B24257
• EC 3.4.22
• EC 3.4.22.16
• MGC1519
• minichain
• N-benzoylarginine-beta-naphthylamide hydrolase

Background

Cathepsin H is a lysosomal cysteine protease of the papain family (1). It is synthesized as a precursor protein, consisting of a signal peptide (residues 1‑20), a propeptide (residues 21‑95), a mini chain (residues 96‑103), a heavy chain (residues 114‑290) and a light chain (residues 291‑333) (2, 3). A truncated form with a 12 amino acid deletion in the signal peptide region is secreted (4). Cathepsin H is the only known mono-aminopeptidase in the papain family (5). Cathepsin H expression is significantly increased in prostate tumors, sera of asthmatic patients, and mucosa of colorectal cancer patients (4, 6, 7).

1. Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1089, Academic Press, San Diego. 
2. Lafuse, W.P. et al. (1995) J. Leukoc. Biol. 57:663.
3. Soderstrom, M. et al. (1999) Biochim. Biophys. Acta 1446:35.
4. Waghray, A. et al. (2002) J. Biol. Chem. 277:11533.
5. Guncar, G. et al. (1998) Structure 6:51.
6. Cimerman, N. et al. (2001) Clin. Chim. Acta 310:113.
7. del Re, E.C. et al. (2000) Br. J. Cancer. 82:1317.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.