Human Cathepsin B ELISA Kit (Colorimetric)

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Summary
Product Discontinued
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Order Details


    • Catalog Number
      KA0552
    • Availability
      Product Discontinued

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Human Cathepsin B ELISA Kit (Colorimetric) Summary

Description
CTSB (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human CTSB.
Standard Curve Range
156 to 10000 pg/mL
Sensitivity
<5 pg/mL
Assay Type
Sandwich ELISA
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CTSB

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Application Notes
Useful for quantification.
Publications
Read Publications using KA0552.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Kit Components

Components
  1. 96-well plate precoated with anti- human Cathepsin B antibody
  2. ABC diluent buffer
  3. Adhesive cover
  4. Antibody diluent buffer
  5. Avidin-Biotin-Peroxidase Complex (ABC), dilution 1:100
  6. Biotinylated anti-human Cathepsin B antibody: dilution 1:100
  7. Lyophilized recombinant human Cathepsin B standard
  8. Sample diluent buffer
  9. TMB color developing agent
  10. TMB stop solution

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Human Cathepsin B ELISA Kit (Colorimetric)

  • APP secretase
  • APPS
  • Cathepsin B
  • Cathepsin B1
  • CPSBamyloid precursor protein secretase
  • CTSB
  • cysteine protease
  • EC 3.4.22
  • EC 3.4.22.1

Background

The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S and O. The aspartyl protease class is composed of cathepsins D and E. cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. cathepsin B is expressed in luminal epithelial cells, indicating that cathepsin B is a marker for secretory cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Publications for Cathepsin B ELISA Kit (KA0552)(3)

Reviews for Cathepsin B ELISA Kit (KA0552) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Cathepsin B ELISA Kit (KA0552). (Showing 1 - 2 of 2 FAQs).

  1. What's the difference between the Cathepsin B DuoSet ELISA (DY2176) and the other Cathepsin B ELISA (KA0552)? The DuoSet ELISA is much cheaper per plate.
    • The duoset contains just the antibodies.
  2. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Additional Cathepsin B Products

Research Areas for Cathepsin B ELISA Kit (KA0552)

Find related products by research area.

Blogs on Cathepsin B.

Cathepsin B - a lysosomal protease with potential of an important drug target in neurological diseases and cancer
Cathepsins are a family of lysosomal proteases (serine, aspartic and cysteine proteases) that acts in conjunction with lipases and nucleases to degrade biological macromolecules in the lysosomes (1). While most cathepsins are ubiquitously expressed...  Read full blog post.

Caspase 11: A novel non-canonical inflammasomes
Cell death via apoptosis is a key cellular function triggered by the cell death receptor family and their ligands. This regulated process then transmits downstream signals through adaptor molecules ending with the caspase cysteine proteases. Caspas...  Read full blog post.

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Bioinformatics

Gene Symbol CTSB