Cathepsin B Antibody (S08-7H4)

Western Blot: Cathepsin B Antibody (S08-7H4) [NBP3-14882] - Western blot detection of Cathepsin B in Rat Brain, C6, 3T3 cell lysates using Cathepsin B Rabbit mAb (1:1000 diluted). Predicted band size: 37kDa. Observed band size: 44, 27, 24kDa.

Immunocytochemistry/Immunofluorescence: Cathepsin B Antibody (S08-7H4) [NBP3-14882] - Immunofluorescence of Cathepsin B (green) in Raw264. 7 using NBP3-14882 at dilution 1/50, and DAPI (blue)

• Reactivity: Mu, Rt
• Applications: WB
• Clone: S08-7H4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Cathepsin B Antibody (S08-7H4) Summary

• Description: Novus Biologicals Rabbit Cathepsin B Antibody (S08-7H4) (NBP3-14882) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein of mouse Cathepsin B (Uniprot#: P10605)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: Ctsb
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1/1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for Cathepsin B Antibody (S08-7H4)

• APP secretase
• APPS
• Cathepsin B
• Cathepsin B1
• CPSBamyloid precursor protein secretase
• CTSB
• cysteine protease
• EC 3.4.22
• EC 3.4.22.1

Background

The cathepsin family of proteolytic enzymes contains several diverse classes of proteases. The cysteine protease class comprises cathepsins B, L, H, K, S and O. The aspartyl protease class is composed of cathepsins D and E. cathepsin G is in the serine protease class. Most cathepsins are lysosomal and each is involved in cellular metabolism, participating in various events such as peptide biosynthesis and protein degradation. cathepsin B is expressed in luminal epithelial cells, indicating that cathepsin B is a marker for secretory cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: Ctsb