Cathepsin B: Novus Biologicals

AF965

Cathepsin B Antibody [Unconjugated]

(1 Review)

(58 Publications)

100 μg

AF965

Cathepsin B was detected in perfusion fixed frozen sections of mouse thymus using 5 µg/mL Goat Anti-Mouse Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF965) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

<b>Biological Strategies Validation. </b>EMT in BSA-induced damaged tubule was associated with increased levels of DPP-4, integrin beta 1 and CAV1; TENE treatment ameliorated these alterations. (a–e) Multiplex immunofluorescence microscopy analysis of the EMT program and association with CAV1. Formaldehyde-fixed, paraffin-embedded (FFPE) kidney samples were labeled with epithelial markers for E-cadherin, alpha SMA and CAV1. An immunofluorescence analysis was performed by confocal microscopy. (d) The enlarged image of the inset shown in (c). The alpha SMA-positive damaged tubular cells were surrounded by alpha SMA-positive interstitial cells (f–j). Multiplex immunofluorescence was performed to analyze the crosstalk among DPP-4, integrin beta 1 and CAV1 in the BSA-injected diabetic mice. (i) The enlarged image of the inset shown in (h). DPP-4, integrin beta 1, and CAV1 were localized at the same location (likely the luminal side of the proximal tubule). The crosstalk occurred more frequently in the damaged tubular cells. Representative images from n = 7 in each group are shown. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31101909), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>BSA-injected diabetic mice exhibited high tubular levels of DPP-4, CAV1 and EMT program; TENE treatment ameliorated these alterations. Immunohistochemical analysis of (a–d) DPP-4, (e–h) CAV1, (i–l) snail and (m–p) AQP-1 from the BSA-injected control or diabetic mice with or without the TENE treatment. Scale bar, 50 μm. Representative images from n = 7 in each group are shown. Each group was analyzed with an unpaired two-tailed t-test. *P < 0.05, **P < 0.01. Data are presented as mean ± s.e.m (q–t). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31101909), licensed under a CC-BY license. Not internally tested by R&D Systems.

Alteration of the size of lysosomes and the expression of LAMP-1 in in Rab7 delta pan pancreatic acinar cells. (a–d) Immunofluorescence images of wild (a,c) and Rab7 delta pan (b,d) pancreases stained with anti-LAMP1(a,b) or anti-cathepsin B (c,d) antibodies (red). DAPI was used for nuclear staining (blue). Bars: 20 µm. (e) Quantification of the positive signals in immunofluorescence images of LAMP-1 (left panel) and cathepsin B (right panel). *P < 0.05. (f) WB of LAMP-1 using total pancreas homogenate of wild and Rab7 delta pan mice. An antibody against LAMP-1 N-terminal (top panel) and an antibody against LAMP-1 C-terminal (middle panel) were utilized. Anti-LAMP-1 N-terminal antibody revealed the shifting of intense bands to a lower position (top panel, arrow head) than that of full-length LAMP-1 (120 kDa) in Rab7 delta pan pancreas. In contrast, anti-LAMP-1 C-terminal antibody revealed bands at 120 kDa only in both wild and Rab7 delta pan pancreas (middle panel). beta -actin was used as an internal loading control (bottom panel). Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/s41598-017-02988-3), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>TENE treatment suppressed the crosstalk among DPP-4, integrin beta 1 and CAV1 via inhibition of TGF-beta /smad3 signaling pathway in vitro. Duolink in situ analysis of (a-c) DPP-4/integrin beta 1, (d–f) DPP-4/CAV1 and (g–i) integrin beta 1/CAV1 in HK-2 cells with or without TGF-beta 1 (10 ng/ml) was performed by confocal microscopy (×1260). Scale bar: 50 μm in each panel. (j) Representative western blot analysis. As a densitometric analysis, each protein level was normalized with actin. n = 6 per group were analyzed. (k–n) Duolink in situ analysis of integrin beta 1/CAV1 in DPP-4 overexpressed HK-2 cells with or without TENE and SIS3. (o) Immunoprecipitation analysis revealed TGF-beta treatment increased crosstalk among DPP-4, integrin beta 1 (ITG beta 1) and CAV1. (p) Immunoprecipitation assay revealed TGF-beta neutralization suppressed crosstalk among DPP-4, integrin beta 1 and CAV1 induced by DPP-4 overexpression. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31101909), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Myc induces cathepsin L expression in beta-cells of pancreatic Islets.(A) Immunohistochemical analyses for CTS B, C, L or S expression (all in red) in combination with staining for the pan-leukocyte marker CD45 (green) in pancreatic islet tumors from the MycERTAM;Bcl-xL animals. Pancreata were harvested from the MycERTAM;Bcl-xL mice treated for 7 d with TAM (Myc-On, 7 days) or control vehicle in place of TAM (Myc-OFF). The islet area is indicated by dotted line. The asterisks indicate the area of tumor represented in the insets. The panels are representatives of at least three animals assayed at each data point, all immunohistochemical analyses were done in duplicate; eight randomized fields per analysis were examined. Scale bars, 100μm. (B) Immunohistochemical analysis for cathepsin L expression in beta-cells of pancreatic islets from MycERTAM;Bcl-xL animals identified by insulin expression. Pancreata were collected from the animals described above. Scale bars represent 25μm. The panels are representatives of three animals assayed at each data point, all immunohistochemical analyses were done in duplicate; ten randomized fields per analysis were examined. Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0120348), licensed under a CC-BY license. Not internally tested by R&D Systems.

Evaluation of lysosomal activation in SWCNT-exposed cells. Panel a: Epi-fluorescence images of macrophages unexposed (upper line) or exposed (lower line) to 50 μg/ml SWCNT for 24 hours stained with Acridine Orange (for lysosomes, in red) and DAPI (RNA/DNA, in green). Merge: combination of Acridine Orange and DAPI staining (overlapping appeared yellow). Panel b: Cathepsin activity in macrophages exposed to SWCNT for 3 hours. *: p < 0.001 between groups. Panel c: Western Blot images of LAMP-1 (120 kDa) and Cathepsin B (37 kDa) expression in macrophages exposed for 24 hours to SWCNT. beta -Actin is given as internal standard. Panel d: quantification of LAMP-1 and Cathepsin B expression, normalized to beta -Actin expression. *: p < 0.05 between groups. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/23800198), licensed under a CC-BY license. Not internally tested by R&D Systems.

Distinct lysosomal phenotypes among different types of neurons in the cerebellum of young Tmem106b−/− mice. a, b Immunostaining of PVALB and Cathepsin D (Cath D) in cerebellar sections from 2-month‐old WT and Tmem106b−/− mice. Representative images from the molecular cell layer are shown. The intensity of Cath D in PVALB-positive interneurons was quantified in b. n = 3. ****, p < 0.0001, unpaired t-test. Scale bar = 10 μm. c, d Immunostaining of Cathepsin D (Cath D) and Hoechst in cerebellar sections from 2-month‐old WT and Tmem106b−/− mice, and images were captured from granule cell layer. The intensity of Cath D in the granule cell layer was quantified in d. n = 3. **, p < 0.001, unpaired t-test. Scale bar = 10 μm. e, f Immunostaining of Cathepsin D (Cath D) and MAP2 in cerebellar sections from 2-month‐old WT and Tmem106b−/− mice. Representative images from the DCN region are shown. The intensity of Cath D in MAP2-positive DCN neurons was quantified in f. Zoom-in image shows the enlarged lysosomes in 2-month‐old Tmem106b−/− mice compared with WT mice. n = 3. ****, p < 0.0001, unpaired t-test. Scale bar = 10 μm Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35287730), licensed under a CC-BY license. Not internally tested by R&D Systems.

CatB is expressed highly in the developing mouse cortex. A CatB protein levels in mouse cortex at indicated embryonic (E), postnatal (P), or adult stages assessed by Western blot. Representative blots from three independent experiments (n = 3 mice per time point). B, C Quantification of pro-form (B) and mature-form (C) CatB expression normalized to Actin (n = 3 mice per group). D Schematic illustration of cell migration from ventricular zone (VZ) to preplate (PP) in E12.5 embryos. E Fluorescent images of CatB (magenta) and Hoechst (blue) in WT mice at E12.5, E14.5 and P0. Scale bar, 100 μm. F Quantification of CatB staining intensity in (E), normalized to VZ/SVZ at each time point. Images were taken from 3 mice per stage, 3 brain sections per mouse. G Immunofluorescent staining of CatB (magenta) with Ctip2 (green) or Trb1(green) in the cortex of WT mice at E12.5. Scale bar, 50 μm. *P < 0.05, **P < 0.01. Error bars represent mean ± SEM. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40702210), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF965U-100UG
Alexa Fluor 405	AF965V-100UG
Alexa Fluor 488	AF965G-100UG
Alexa Fluor 532	AF965X-100UG
Alexa Fluor 594	AF965T-100UG
Alexa Fluor 647	AF965R-100UG
Alexa Fluor 700	AF965N-100UG
Alexa Fluor 750	AF965S-100UG
Alexa Fluor Plus 405	AF965AFP405-100UG
Alexa Fluor Plus 488	AF965AFP488-100UG
Alexa Fluor Plus 555	AF965AFP555-100UG
Alexa Fluor Plus 594	AF965AFP594-100UG
Alexa Fluor Plus 647	AF965AFP647-100UG
Alexa Fluor Plus 680	AF965AFP680-100UG
Allophycocyanin	AF965A
Biotin	BAF965
CoraFluor 1	AF965CL1
DyLight 350	AF965D
DyLight 405	AF965E
DyLight 488	AF965K
DyLight 550	AF965L
DyLight 594	AF965M
DyLight 650	AF965W
DyLight 680	AF965Y
DyLight 755	AF965Z
FITC	AF965F
HRP	AF965H
Janelia Fluor 525	AF965JF525
Janelia Fluor 549	AF965I
Janelia Fluor 646	AF965J
Janelia Fluor 669	AF965JF669
PE	AF965P
PerCP	AF965C
mFluor Violet 450 SE	AF965MFV450
mFluor Violet 500 SE	AF965MFV500
mFluor Violet 610 SE	AF965MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF965-SP

compare

(select up to 3 products from this page to compare)

AF953

Cathepsin B Antibody [Unconjugated]

(33 Publications)

100 μg

AF953

HAF019). A specific band was detected for Cathepsin B at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF019). A specific band was detected for Cathepsin B at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cathepsin B at approximately 25-30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Cathepsin B was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Cathepsin B at approximately 34 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

HAF017). Specific bands were detected for Cathepsin B at approximately 25-45 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. New adjunct appears with knockout cell line." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Cathepsin B knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). Specific bands were detected for Cathepsin B at approximately 25-45 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1. New adjunct appears with knockout cell line." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

Cathepsin B was detected in immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line (Positive) & absent in Daudi human Burkitt's lymphoma cell line (Negative) using Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # <a class=

NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

$<b>Biological Strategies Validation. </b>Legumain, cathepsin B and L expressions in cytosolic and membrane fractions of myotubes.Myotubes were treated with (+) or without (−) 30 µM simvastatin for 48 h before subcellular fractionation was performed. One representative immunoblot of legumain, cathepsin B and L in the cytosolic and membrane fractions is shown. All lanes were loaded with equal amount of total proteins and probed with antibodies as indicated. LAMP-2 and alpha -tubulin are shown as cell compartment controls (n = 3). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24416446), licensed under a CC-BY license. Not internally tested by R&D Systems.$

<b>Genetic Strategies Validation. </b>CTSS inhibition does not impair lysosomal activities.(a) OEC-M1 cells were pretreated with vesicle or 100 nM BAF for 1 h and then incubated with 20 μM 6r for 1 h further. Lysosomal proteolytic activities were determined using BODIPY–BSA and quantified through flow cytometry. (b) After 48 h of siRNA knockdown of CTSS and CTSB, the relative expression of CTSS and CTSB were determined through Western blotting (right panel). Lysosomal proteolytic activities were determined using BODIPY–BSA and quantified through flow cytometry (right panel). Data represent the mean ± SD of three independent experiments. Differences were found to be statistically significant at ***P < 0.001. Image collected and cropped by CiteAb from the following open publication (//www.nature.com/articles/srep29256), licensed under a CC-BY license. Not internally tested by R&D Systems.

$HAP1 cell line lysates were prepared and immunoprecipitation was performed using 2.0 µg of Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) pre-coupled to Dynabeads Protein A. Immunoprecipitated Cathepsin B was detected in Western Blot with a rabbit anti-Cathepsin B antibody used at 1/500. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).$

953-CY) was serially diluted and captured by Mouse Anti-Human Pro Cathepsin B Monoclonal Antibody (Catalog # MAB2176) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human Cathepsin B (Catalog # <a class=

953-CY) was serially diluted and captured by Mouse Anti-Human Pro Cathepsin B Monoclonal Antibody (Catalog # MAB2176) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF953U-100UG
Alexa Fluor 405	AF953V-100UG
Alexa Fluor 488	AF953G-100UG
Alexa Fluor 532	AF953X-100UG
Alexa Fluor 594	AF953T-100UG
Alexa Fluor 647	AF953R-100UG
Alexa Fluor 700	AF953N-100UG
Alexa Fluor 750	AF953S-100UG
Alexa Fluor Plus 405	AF953AFP405-100UG
Alexa Fluor Plus 488	AF953AFP488-100UG
Alexa Fluor Plus 555	AF953AFP555-100UG
Alexa Fluor Plus 594	AF953AFP594-100UG
Alexa Fluor Plus 647	AF953AFP647-100UG
Alexa Fluor Plus 680	AF953AFP680-100UG
Allophycocyanin	AF953A
Biotin	BAF953
CoraFluor 1	AF953CL1
DyLight 350	AF953D
DyLight 405	AF953E
DyLight 488	AF953K
DyLight 550	AF953L
DyLight 594	AF953M
DyLight 650	AF953W
DyLight 680	AF953Y
DyLight 755	AF953Z
FITC	AF953F
HRP	AF953H
Janelia Fluor 525	AF953JF525
Janelia Fluor 549	AF953I
Janelia Fluor 646	AF953J
Janelia Fluor 669	AF953JF669
PE	AF953P
PerCP	AF953C
mFluor Violet 450 SE	AF953MFV450
mFluor Violet 500 SE	AF953MFV500
mFluor Violet 610 SE	AF953MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF953-SP

compare

(select up to 3 products from this page to compare)

953-CY

Recombinant Human Cathepsin B Protein, CF

(22 Publications)

10 μg

953-CY

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

DCATB0

Human Pro-Cathepsin B Quantikine ELISA Kit

(9 Publications)

1 Kit

DCATB0

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

DY2176

Human Total Cathepsin B DuoSet ELISA, 15 Plate

(9 Publications)

1 Kit (for 15 Plates)

DY2176

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

965-CY

Recombinant Mouse Cathepsin B Protein, CF

(8 Publications)

10 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

compare

(select up to 3 products from this page to compare)

MAB965

Cathepsin B Antibody (173317)

(6 Publications)

500 μg

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB965U-100UG
Alexa Fluor 405	FAB965V-100UG
Alexa Fluor 488	FAB965G-100UG
Alexa Fluor 532	FAB965X-100UG
Alexa Fluor 594	FAB965T-100UG
Alexa Fluor 647	FAB965R-100UG
Alexa Fluor 700	FAB965N-100UG
Alexa Fluor 750	FAB965S-100UG
Alexa Fluor Plus 405	FAB965AFP405-100UG
Alexa Fluor Plus 488	FAB965AFP488-100UG
Alexa Fluor Plus 555	FAB965AFP555-100UG
Alexa Fluor Plus 594	FAB965AFP594-100UG
Alexa Fluor Plus 647	FAB965AFP647-100UG
Alexa Fluor Plus 680	FAB965AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB965-SP

compare

(select up to 3 products from this page to compare)

MAB2176

Cathepsin B Antibody (155714) [Unconjugated] - Pro form

(1 Publication)

500 μg

MAB2176

HAP1 parental cell line WT and Cathepsin B HAP1 KO cells were labelled with a green or a far-red fluorescent dye, respectively. Cells were stained with Mouse Anti-Human Pro Cathepsin B Monoclonal Antibody (Catalog # MAB2176) followed by incubation with an Alexa-fluor 555 conjugated secondary antibody (upper panel). DAPI-only counterstained cells shown on a lower panel. Acquisition of the blue (nucleus-DAPI), green (identification of WT cells), red (antibody staining) and far-red (identification of KO cells) channels was performed. Representative images of the blue and red (grayscale) channels are shown. WT and KO cells are outlined with green and magenta dashed line, respectively. Primary antibody dilution used: 1 µg/mL. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

953-CY) was serially diluted and captured by Mouse Anti-Human Pro Cathepsin B Monoclonal Antibody (Catalog # MAB2176) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

<b>Genetic Strategies Validation. </b>Recombinant Human Cathepsin B (Catalog # <a class=

953-CY) was serially diluted and captured by Mouse Anti-Human Pro Cathepsin B Monoclonal Antibody (Catalog # MAB2176) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human Cathepsin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF953) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2176U-100UG
Alexa Fluor 405	FAB2176V-100UG
Alexa Fluor 488	FAB2176G-100UG
Alexa Fluor 532	FAB2176X-100UG
Alexa Fluor 594	FAB2176T-100UG
Alexa Fluor 647	FAB2176R-100UG
Alexa Fluor 700	FAB2176N-100UG
Alexa Fluor 750	FAB2176S-100UG
Alexa Fluor Plus 405	FAB2176AFP405-100UG
Alexa Fluor Plus 488	FAB2176AFP488-100UG
Alexa Fluor Plus 555	FAB2176AFP555-100UG
Alexa Fluor Plus 594	FAB2176AFP594-100UG
Alexa Fluor Plus 647	FAB2176AFP647-100UG
Alexa Fluor Plus 680	FAB2176AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2176-SP

compare

(select up to 3 products from this page to compare)

NBP1-86048

Cathepsin B Antibody - BSA Free

(2 Publications)

0.1 ml

NBP1-86048

Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining of human thyroid gland shows moderate granular cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining of human cerebral cortex shows granular cytoplasmic positivity in neuronal cells.

Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining of human lung shows cytoplasmic positivity in macrophages.

Immunohistochemistry-Paraffin: Cathepsin B Antibody [NBP1-86048] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-86048-25ul

compare

(select up to 3 products from this page to compare)

NBP3-15614

Cathepsin B Antibody (6I5D7)

100 ul

NBP3-15614

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

compare

(select up to 3 products from this page to compare)

NBP2-90402

Cathepsin B Antibody (010) - Azide and BSA Free

(3 Publications)

100 ul

NBP2-90402

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-90402AF350
Alexa Fluor 405	NBP2-90402AF405
Alexa Fluor 488	NBP2-90402AF488
Alexa Fluor 532	NBP2-90402AF532
Alexa Fluor 594	NBP2-90402AF594
Alexa Fluor 647	NBP2-90402AF647
Alexa Fluor 700	NBP2-90402AF700
Alexa Fluor 750	NBP2-90402AF750
Alexa Fluor Plus 405	NBP2-90402AFP405
Alexa Fluor Plus 488	NBP2-90402AFP488
Alexa Fluor Plus 555	NBP2-90402AFP555
Alexa Fluor Plus 594	NBP2-90402AFP594
Alexa Fluor Plus 647	NBP2-90402AFP647
Alexa Fluor Plus 680	NBP2-90402AFP680
Allophycocyanin	NBP2-90402APC
Biotin	NBP2-90402B
CoraFluor 1	NBP2-90402CL1
DyLight 350	NBP2-90402UV
DyLight 405	NBP2-90402V
DyLight 488	NBP2-90402G
DyLight 550	NBP2-90402R
DyLight 594	NBP2-90402DL594
DyLight 650	NBP2-90402C
DyLight 680	NBP2-90402FR
DyLight 755	NBP2-90402IR
FITC	NBP2-90402F
HRP	NBP2-90402H
Janelia Fluor 525	NBP2-90402JF525
Janelia Fluor 549	NBP2-90402JF549
Janelia Fluor 646	NBP2-90402JF646
Janelia Fluor 669	NBP2-90402JF669
PE	NBP2-90402PE
PerCP	NBP2-90402PCP
mFluor Violet 450 SE	NBP2-90402MFV450
mFluor Violet 500 SE	NBP2-90402MFV500
mFluor Violet 610 SE	NBP2-90402MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-90402-50ul

compare

(select up to 3 products from this page to compare)

NBP2-67254

Mouse Cathepsin B ELISA Kit (Colorimetric)

1 Kit

NBP2-67254

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Standard Curve:

0.16 - 10 ng/mL

Sensitivity:

0.10 ng/mL

Sample Type:

Serum, Plasma, Urine, Cell Supernatant, Tissue Homogenate

compare

(select up to 3 products from this page to compare)

At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Ctsb Products By Gene Name

Cathepsin B Antibody [Unconjugated]

Cathepsin B Antibody [Unconjugated]

Recombinant Human Cathepsin B Protein, CF

Human Pro-Cathepsin B Quantikine ELISA Kit

Human Total Cathepsin B DuoSet ELISA, 15 Plate

Recombinant Mouse Cathepsin B Protein, CF

Cathepsin B Antibody (173317)

Cathepsin B Antibody (155714) [Unconjugated] - Pro form

Cathepsin B Antibody - BSA Free

Cathepsin B Antibody (6I5D7)

Cathepsin B Antibody (010) - Azide and BSA Free

Mouse Cathepsin B ELISA Kit (Colorimetric)