Catenin alpha 1 Antibody

Western Blot: Catenin alpha 1 Antibody [NB100-2917] - Analysis of Catenin alpha 1 using NB100-2917 at 1ug/mL in human colon lysate (35 ug protein in RIPA buffer).

Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NB100-2917] - Staining of paraffin embedded human uterus. Antibody at 3.75 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: Catenin alpha 1 Antibody [NB100-2917] - HEK293 overexpressing Human CTNNA1 and probed with (mock transfection in first lane).

Immunohistochemistry-Paraffin: Catenin alpha 1 Antibody [NB100-2917] - Staining of paraffin embedded human prostate. Image shows staining of cell membranes of the secretory cells in the glands. Antibody at 3.8 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Catenin alpha 1 Antibody Summary

• Immunogen: Peptide with sequence C-KREKQDETQTKIK corresponding to internal region according to NP_001894.2.
• Epitope: C-KREKQDETQTKIK
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CTNNA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/mL
  • Immunohistochemistry-Paraffin 3.75 ug/mL
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 1 - 3 ug/mL
• Application Notes: WB: Approx. 100 kDa band observed in human colon and human skin lysates (calculated MW of 100 kDa band according to NP_001894.2).
• Control:

  Catenin alpha 1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Catenin alpha 1 Antibody

• Alpha E-catenin
• alpha-catenin
• alpha-E-catenin
• alphaE-catenin
• cadherin-associated protein
• CAP102
• catenin (cadherin-associated protein), alpha 1 (102kD)
• catenin (cadherin-associated protein), alpha 1, 102kDa
• catenin alpha-1
• FLJ36832
• FLJ52416
• Renal carcinoma antigen NY-REN-13

Background

The centaurin alpha 1 protein is a high affinity PtdIns(3,4,5)P3 binding protein enriched in brain. By acting as a GTPase activating protein for ADP ribosylation factor 6 (ARF6), centaurin alpha 1 is able to switch off ARF6 and inhibit its ability to mediate beta 2 adrenoceptor internalization and negatively regulates ARF6 activity by functioning as an in vivo PIP3 dependent ARF6 GAP.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Catenin alpha 1 Antibody (NB100-2917)(1)

Publication using NB100-2917

• Lynch HT, Grady W, Suriano G, Huntsman D. Gastric cancer: New genetic developments. J Surg Oncol 2005-05-13 [PMID: 15895459]

Bioinformatics

• Gene Symbol: CTNNA1
• Entrez:
  • 1495(Human)
  • 12385(Mouse)
  • 307505(Rat)
• Uniprot:
  • NP_001894.2(Human)