Catalase Antibody

Product Discontinued

Catalase Antibody Summary

• Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human catalase was used as an immunogen.
• Marker: Peroxisome Marker
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAT
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:100-250
  • Immunoprecipitation 1:10
  • Western Blot 1:1000
• Application Notes: This product is useful for: Western Blot, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunoprecipitation. In Western blot this antibody detects a band at approximately 60kDa.
• Theoretical MW: 60 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Catalase Overexpression Lysate

Reactivity Notes

Reacts in Human, Mouse, Rat.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 49% PBS, 0.05% BSA and 50% Glycerol
• Preservative: 0.01% Sodium Azide
• Purity: IgG purified

Alternate Names for Catalase Antibody

• Cas1
• CAT
• Catalase
• Cs-1
• EC 1.11.1.6
• MGC138422
• MGC138424

Background

Catalase is a tetrameric hemoprotein which degrades H2O2. Human catalase is a heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer (1). Although catalase is a major intracellular antioxidant, the expression of the human catalase gene appears to be limited in the airway epithelium, making these cells vulnerable to oxidant stress (2). Transgenic mice that overexpress human catalase localized to the peroxisome, the nucleus, or mitochondria showed cardiac pathology and cataract development were delayed, oxidative damage was reduced, H2O2 production and H2O2-induced aconitase inactivation were attenuated, and the development of mitochondrial deletions was reduced (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Catalase Antibody (NB100-79910)(9)

Publications using NB100-79910

• Son YO, Pratheeshkumar P, Divya SP et al. Nuclear factor erythroid 2-related factor 2 enhances carcinogenesis by suppressing apoptosis and promoting autophagy in nickel-transformed cells. J. Biol. Chem. 2017-03-22 [PMID: 28330870] (WB)
• Lee WH, Higuchi H, Ikeda S et al. Mouse Tmem135 mutation reveals a mechanism involving mitochondrial dynamics that leads to age-dependent retinal pathologies. Elife. 2016-11-15 [PMID: 27863209] (WB, Mouse)
• Son YO, Poyil P, Roy RV et al. Nrf2/p62 Signaling in Apoptosis Resistance and Its Role in Cadmium-induced Carcinogenesis J. Biol. Chem. 2014-08-25 [PMID: 25157103]
• Quintero M, Gonzalez-Martin MD, Vega-Agapito V et al. The effects of intermittent hypoxia on redox status, NFkB activation, and plasma lipid levels are dependent on the lowest oxygen saturation. Free Radic Biol Med. 2013-08-31 [PMID: 24002010] (WB, Rat)
• Chan JK, Charrier JG, Kodani SD et al. Combustion-derived flame generated ultrafine soot generates reactive oxygen species and activates Nrf2 antioxidants differently in neonatal and adult rat lungs. Part Fibre Toxicol 2013-08-01 [PMID: 23902943] (WB, Rat)
• Mitropoulos D, Patris E, Deliconstantinos G et al. Terazosin-induced alterations in catalase expression and lipid peroxidation in the rat seminal vesicles Andrologia 2012-06-26 [PMID: 22731390] (IF/IHC, Rat)
• Wang X, Mandal AK, Saito H, Pulliam JF, Lee EY, Ke ZJ, Lu J, Ding S, Li L, Shelton BJ, Tucker T, Evers BM, Zhang Z, Shi X. Arsenic and chromium in drinking water promote tumorigenesis in a mouse colitis-associated colorectal cancer model and the potential mechanism is ROS-mediated Wnt/beta-catenin signaling pathway. Toxicol Appl Pharmacol. 2012-04-19 [PMID: 22552367] (WB, Mouse)
• Wang X, Son YO, Chang Q, Sun L, Hitron JA, Budhraja A, Zhang Z, Ke Z, Chen F, Luo J, Shi X. NADPH oxidase activation is required in reactive oxygen species generation and cell transformation induced by hexavalent chromium. Toxicol Sci;123(2):399-410. 2011-10-01 [PMID: 21742780] (WB, Human)
• Toutzaris D, Lewerenz J, Albrecht P et al. A novel giant peroxisomal superoxide dismutase motif-containing protein. Free Radic Biol Med 2010-03-01 [PMID: 20045724]

Bioinformatics

• Gene Symbol: CAT
• Entrez:
  • 847(Human)
  • 12359(Mouse)
  • 24248(Rat)
• Uniprot:
  • P04040()