Catalase Antibody (JM11-12)

Western Blot: Catalase Antibody (JM11-12) [NBP2-67212] - Analysis of Catalase on different cells lysates using anti-Catalase antibody at 1/500 dilution. Positive control: Line 1: Zebrafish Line 2: Mouse brain Line 3: HepG2

Immunohistochemistry-Paraffin: Catalase Antibody (JM11-12) [NBP2-67212] - Analysis of paraffin-embedded rat adrenal gland tissue using anti-Catalase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Catalase Antibody (JM11-12) [NBP2-67212] - Analysis of paraffin-embedded human liver tissue using anti-Catalase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Catalase Antibody (JM11-12) [NBP2-67212] - Analysis of paraffin-embedded human uterus tissue using anti-Catalase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Catalase Antibody (JM11-12) [NBP2-67212] - Analysis of paraffin-embedded mouse liver tissue using anti-Catalase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Catalase Antibody (JM11-12) [NBP2-67212] - Analysis of paraffin-embedded mouse prostate tissue using anti-Catalase antibody. Counter stained with hematoxylin.

Product Discontinued

Catalase Antibody (JM11-12) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human Catalase aa 51-100. (SwissProt: P04040 Human; SwissProt: P24270 Mouse; SwissProt: P04762 Rat)
• Localization: Peroxisome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CAT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Catalase Antibody (JM11-12)

• Cas1
• CAT
• Catalase
• Cs-1
• EC 1.11.1.6
• MGC138422
• MGC138424

Background

Human catalase is an heme-containing peroxisomal enzyme that breaks down hydrogen peroxide to water and oxygen; it is implicated in ethanol metabolism, inflammation, apoptosis, aging and cancer. The 1.5A resolution human enzyme structure, both with and without bound NADPH, establishes the conserved features of mammalian catalase fold and assembly, implicates Tyr370 as the tyrosine radical, suggests the structural basis for redox-sensitive binding of cognate mRNA via the catalase NADPH binding site, and identifies an unexpectedly substantial number of water-mediated domain contacts (1). Because catalase has a low affinity for H2O2, it has been suggested that glutathione peroxidase clears most H2O2 within the erthrocyte and that catalase is of little import. It has been hypothesized that erythrocyte catalase might function to protect heterologous somatic cells against challenge by high levels of exogenous H2O2 (e.g., in areas of inflamation) (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAT