Caspr2 Antibody [Unconjugated]

Western blot shows lysates of human brain tissue, mouse brain tissue, and rat brain tissue. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat Caspr2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5145) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Caspr2 at approximately 160-170 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Caspr2 was detected in perfusion fixed frozen sections of mouse brain using 10 µg/mL Sheep Anti-Human/Mouse/Rat Caspr2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5145) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

Caspr2 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Caspr2 isoform 1
  Pro29-Ala1262
  Accession # Q9UHC6
• Specificity: Detects human Caspr2 in direct ELISAs and human, mouse, and rat Caspr2 in Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Caspr1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: CNTNAP2
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspr2 Antibody [Unconjugated]

• AUTS15
• Caspr2
• CASPR2DKFZp781D1846
• CDFE
• Cell recognition molecule Caspr2
• CNTNAP2
• contactin associated protein-like 2
• contactin-associated protein 2
• contactin-associated protein-like 2
• homolog of Drosophila neurexin IV
• KIAA0868
• NRXN4
• NRXN4Caspr2
• PTHSL1

Background

Caspr2 (CNTNAP2, Contactin-associated protein-like 2 and Cell recognition molecule Caspr2) is a 160-170 kDa glycoprotein, a member of the neurexin family of proteins.  It is predominantly expressed in nervous system and likely play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the juxtaparanodal region of the axo-glial junction. Caspr2 and closely related molecule Caspr/Paranodin demarcate distinct subdomains in myelinated axons. While contactin-associated protein (Caspr) is present at the paranodal junctions, Caspr2 is precisely colocalized with Shaker-like K+ channels in the juxtaparanodal region.  Over aa 29-1262, human CASPR2 shares 94% aa sequence identity with mouse Caspr2.                    

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspr2 Antibody (AF5145)(2)

Publications using AF5145

• Tang W, Chai W, Du D et al. The lncRNA-AK046375 Upregulates Metallothionein-2 by Sequestering miR-491-5p to Relieve the Brain Oxidative Stress Burden after Traumatic Brain Injury Oxidative medicine and cellular longevity 2/16/2022 [PMID: 35222805]
• Kristina R. Patterson, Josep Dalmau, Eric Lancaster Mechanisms of Caspr2 antibodies in autoimmune encephalitis and neuromyotonia Annals of Neurology 2018-01-06 [PMID: 29244234]

Bioinformatics

• Gene Symbol: CNTNAP2
• Uniprot:
  • Q9UHC6()