Lgi1 Antibody (S283-7)

Images

 
Western Blot: Lgi1 Antibody (S283-7) [NBP2-42190] - Western Blot analysis of Rat Brain Membrane showing detection of ~60 kDa Lgi1 protein using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Load: 10 ...read more
Immunocytochemistry/ Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Immunocytochemistry/ Immunofluorescence analysis using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Tissue: Neuroblastoma ...read more
Immunocytochemistry/ Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Tissue: Neuroblastoma cell line SK-N-BE. Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-LGI1 ...read more
Immunocytochemistry/ Immunofluorescence: Lgi1 Antibody (S283-7) [NBP2-42190] - Immunocytochemistry/ Immunofluorescence analysis using Mouse Anti-Lgi1 Monoclonal Antibody, Clone S283-7 (NBP2-42190). Tissue: Neuroblastoma ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
S283-7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Lgi1 Antibody (S283-7) Summary

Immunogen
Fusion protein amino acids 37-113 (LRRNT domain and first LRR repeat) of mouse LGI1. Rat: 100% identity (77/77 amino acids identical). Human: 98% identity (76/77 amino acids identical). ~50% identity with LGI2, LGI3 and LGI4.
Localization
Cell Junction , Golgi Apparatus , Endoplasmic Reticulum
Specificity
approx 50% identity with LGI2, LGI3 and LGI4.Detects approx 60kDa.
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
LGI1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Western Blot 1:1000
Application Notes
1 ug/ml of LGI1 Antibody was sufficient for detection of LGI1 in 20 ug of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.

Reactivity Notes

Immunogen displays sequence identity for non-tested species: Human and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4), 50% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Lgi1 Antibody (S283-7)

  • ADLTE
  • ADPAEF
  • ADPEAF
  • BB130740
  • epilepsy, partial
  • Epitempin
  • Epitempin-1
  • EPT
  • EPTleucine-rich glioma-inactivated protein 1
  • ETL1
  • ETL1epitempin-1
  • IB1099
  • leucine-rich, glioma inactivated 1
  • LGI1

Background

The Lgi1 gene is rearranged as a result of translocations in glioblastoma cell lines. The protein contains a hydrophobicsegment representing a putative transmembrane domain with the amino terminus located outside the cell. It alsocontains leucine-rich repeats

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LGI1
Uniprot