Caspase-9 Activity Assay Kit (Colorimetric)

Caspase-9 Activity Assay Kit (Colorimetric) [NBP2-54823] - Cell lysates from murine insulinoma cell lines. Assay performed as per Novus' protocol.

Product Discontinued

Caspase-9 Activity Assay Kit (Colorimetric) Summary

• Description: Simple and convenient colorimetric assay to measure Caspase-9 activity within 1-2 hrs.
  
  Detection method: Absorbance at 400 or 405 nmApplications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65608)
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: CASP9

Applications/Dilutions

Reviewed Applications

Read 1 Review rated 5 using NBP2-54823 in the following application:

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase-9 Activity Assay Kit (Colorimetric)

• APAF-3APAF3
• apoptotic protease activating factor 3
• Apoptotic protease Mch-6
• Apoptotic protease-activating factor 3
• CASP9
• CASP-9
• caspase 9, apoptosis-related cysteine peptidase
• Caspase9
• Caspase-9
• EC 3.4.22.62
• ICE-LAP6CASPASE-9c
• ICE-like apoptotic protease 6
• MCH6apoptosis-related cysteine protease

Background

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-9 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEHD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Review for Caspase-9 Kit (NBP2-54823) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Francesca Pacifici 12/06/2018

Summary

• Lot: 3L09K01190

Comments

• Comments: Cell lysates from murine insulinoma cell lines. We performed the assay by following manufacturer's protocol.

FAQs for Caspase-9 Kit (NBP2-54823). (Showing 1 - 1 of 1 FAQ).

1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
   • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Bioinformatics

• Gene Symbol: CASP9