Caspase-9 Antibody - (active/cleaved)

Product Discontinued

Caspase-9 Antibody - (active/cleaved) Summary

• Immunogen: Synthetic peptide corresponding to amino-terminal residues adjacent to Asp353 of rat caspase-9.
• Specificity: The anti-active caspase-9 antibody recognizes the cleaved caspase-9 (37 kDa/17 kDa). It does not recognize full-length caspase-9 or any other caspases.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASP9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10-20 ug/ml
  • Western Blot 0.5-4 ug/ml

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 0.5% BSA, and 30% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for Caspase-9 Antibody - (active/cleaved)

• APAF-3APAF3
• apoptotic protease activating factor 3
• Apoptotic protease Mch-6
• Apoptotic protease-activating factor 3
• CASP9
• CASP-9
• caspase 9, apoptosis-related cysteine peptidase
• Caspase9
• Caspase-9
• EC 3.4.22.62
• ICE-LAP6CASPASE-9c
• ICE-like apoptotic protease 6
• MCH6apoptosis-related cysteine protease

Background

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are synthesized as zymogens (inactive pro enzyme precursors which require a biochemical change to become active enzymes) with an N-terminal prodomain of variable length followed by a large subunit (p20) and a small subunit (p10). Caspases are activated through proteolytic cleavage at specific asparagine residues that are located within the prodomain, the p10, and p20 subunits. Activation results in the generation of mature active caspases that consist of the heterotetramer p202-p102. Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. The Active/Cleaved Caspase-9 polyclonal antisera preferntially recognizes active/cleaved caspase-9. Whereas the antisera has a strong preference for active/cleaved caspase-9, in some cell or tissue systems or techniques the antisera may also recognize the proform of caspase-9. On western blots, the proform of caspase-9 is detected at ~50 kDa, the large subunit at ~35 kDa, and the small subunit at ~15 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-9 Antibody (NBP1-45449)(1)

Publication using NBP1-45449

• Udayanga KGS, Miyata H, Yokoo Y et al. Immunohistochemical study of the apoptosis process in epidermal epithelial cells of rats under a physiological condition. Histol Histopathol;26(7):811-820. 2011-07-01 [PMID: 21630211]

FAQs for Caspase-9 Antibody (NBP1-45449). (Showing 1 - 1 of 1 FAQ).

1. Could I use HeLa cell lysate as a positive control for Caspase 9 primary antibodies in WB?
   • After looking through our testing it does appear you should be fine using HeLa as a positive control cell lysate for Caspase 9 in Western Blot, as we have used this sample successfully in our QC tests and shown expression of Caspase 9.

Bioinformatics

• Gene Symbol: CASP9
• Entrez:
  • 842(Human)
• Uniprot:
  • AAK26235.1(Human)