Caspase-8 Antibody (FLICE 4-1-20)

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Western Blot: Caspase-8 Antibody (FLICE 4-1-20) [NB500-208] - CaSki cells were mock-infected or exposed to each of the indicated viruses (MOI ~ 1 pfu/cell) or 10 uM staurosporine and 4 h pi, cellular lysates were ...read more
Western Blot: Caspase 8 Antibody (FLICE 4-1-20) [NB500-208] - Detection of Caspase 8 (59 kDa) from HEK293 cell extract using NB500-208 (1:500). Lanes 1 and 2 contain inactive and active Caspase 8, respectively.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB
Clone
FLICE 4-1-20
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Caspase-8 Antibody (FLICE 4-1-20) Summary

Immunogen
Full-length recombinant human Caspase 8 [UniProt# Q14790]
Localization
Cytoplasmic
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CASP8
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Application Notes
This Caspase 8 (FLICE 4-1-20) antibody is useful for Western Blot where a band is seen at 55 kDa representing the inactive form of Caspase 8 (see protocol for additional information). We also have reports that the antibody detects the active (pro) form of Caspase 8.
Control
293 Whole Cell Lysate
Caspase-8 Overexpression Lysate
Jurkat Staurosporine Treated / Untreated Cell Lysate
Publications
Read Publication using
NB500-208 in the following applications:

  • WB
    1 publication

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 29069785).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Caspase-8 Antibody (FLICE 4-1-20)

  • AIS
  • androgen receptor
  • CASP8
  • Caspase8
  • Caspase-8
  • DHTRTFM
  • Dihydrotestosterone receptorHYSP1
  • HUMARA
  • Mch5
  • NR3C4KD
  • Nuclear receptor subfamily 3 group C member 4
  • SMAX1SBMA
  • spinal and bulbar muscular atrophy

Background

Caspases are evolutionary conserved family of aspartate-specific cystein proteases which are essential part of apoptotic machinery and typically signal in a two-step cascade: 'initiator caspases' with large prodomains (such as caspase-1, -8 and -9) are first recruited into large protein complexes in which they undergo proximity-induced autoactivation and "executioner caspases" such as caspase-3 and -6, caspase-7 which are activated by initiator caspases via freeing of executioner caspases of their short inhibitory prodomain, allowing them to cleave a large set of cellular substrates. Localized in cytoplasm, caspase 7 is a heterotetramer that is consists of two anti-parallel arranged heterodimers, each one formed by a 20 kD (p20) and a 11 kD (p11) subunit, and interacts with BIRC6/bruce. Caspase 7 cleaves/activates SREBPs and poly(ADP-ribose) polymerase (PARP). Its overexpression promotes apoptosis, and it also implicates in inflammation signaling. Therapeutic intervention with caspase-7 has been shown to prove beneficial in conditions where excessive cell death and/or inflammation contribute to diseases e.g. Alzheimer's disease, Huntington's disease, sepsis, rheumatoid arthritis, cancer and NIDDM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Caspase-8 Antibody (NB500-208)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: WB.


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Product General Protocols

View specific protocols for Caspase-8 Antibody (NB500-208): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Caspase-8 Antibody (NB500-208). (Showing 1 - 3 of 3 FAQ).

  1. May we ask the MW difference between the active form and the proform of Caspase 8 while performing WB?
    • In treated cells induced to undergo apoptosis, caspase-8 migrates as a 55/53 kDa (pro-form), 41/42 kDa (a cleaved/active or intermediate form), and 18 kDa (active form). The proform (55/53 kDa) is still seen in treated cells because not all cells undergo apoptosis at once.
  2. May we ask if NB600-1343, NB100-56527 and NB500-208 can recognize both active form and profor
    • Product NB100-56527 recognises both pro and active forms of Caspase 8. NB500-208 recognises only the active form, and NB600-1343 has not been tested for specificity at this time.
  3. Does NB500-208 both recognize human and rat active form of caspase 8 (subunit p18 or subunit p10)? Besides that please let me know if NB500-208 also recognize pro-form caspase 8?
    • Caspase 8 Antibody (FLICE 4-1-20) # NB500-208 antibody does not recognize rat samples. It is human specific and in our QC data it was successfully tested to detect the pro or inactive form of caspase at molecular weight 59kd. However, depending upon the extent of extrinsic apoptotic pathway activation in your cells or tissues, you can expect to see bands of the active form also.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Caspase-8 Antibody (NB500-208)

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Research Areas for Caspase-8 Antibody (NB500-208)

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Blogs on Caspase-8.

The NLRP3 Inflammasome: Macrophage Activator & Pathology Driver
By Victoria Osinski, PhDWhat is the NLRP3 Inflammasome?With its critical role in innate immunity, the nucleotide-binding oligomerization domain-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome ...  Read full blog post.

Pyroptosis: Mechanisms mediating cell death and pro-inflammatory cytokine release
By Victoria OsinskiPyroptosis is an inflammatory form of programmed cell death characterized by the release of pro-inflammatory cytokines IL-1 beta and IL-18.1,10 It is a process distinct from apoptosis and necrosis (T...  Read full blog post.

Caspase 3, the executioner of apoptosis
Caspase-3 enzyme is a member of the family of endoproteases which regulate inflammation and apoptosis signaling networks. Caspase-3 is known as an executioner caspase in apoptosis because of its role in coordinating the destruction of cellular stru...  Read full blog post.

VPS41 - An important regulator of lysosomal trafficking
Membrane fusion is an essential step during the trafficking of endosomes and vesicles throughout the cell. Membrane fusion events are facilitated by multisubunit tethering complexes (MTC) including CORVET and HOPS. These complexes interact with Rab...  Read full blog post.

Caspase-8 - a pro-apoptotic protein with dynamic roles in normal physiology and pathology
Caspases are a family of cysteine-aspartic acid proteases that are responsible for the initiation and execution of apoptosis. Caspase-8 is a 55 kDa protein expressed as an inactive procaspase that resides in the cytosol. Activation of Caspase-8 req...  Read full blog post.

Caspase 8 - a key mediator of apoptosis
Programmed cell death via apoptosis is a key controlled physiological process instigated by the cell death receptor family, their ligands, and the caspase cysteine protease family. All caspases exist in a precursor form that contains a prodomain, a...  Read full blog post.

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Bioinformatics

Gene Symbol CASP8
Entrez
Uniprot