Caspase 5 Antibody (SD203-2)

Immunocytochemistry/Immunofluorescence: Caspase 5 Antibody (SD203-2) [NBP2-67757] - Staining Caspase 5 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Caspase 5 Antibody (SD203-2) [NBP2-67757] - Analysis of paraffin-embedded human liver tissue using anti-Caspase 5 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Caspase 5 Antibody (SD203-2) [NBP2-67757] - Staining Caspase 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Caspase 5 Antibody (SD203-2) [NBP2-67757] - Staining Caspase 5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Caspase 5 Antibody (SD203-2) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Caspase 5 aa 1-50 / 434. (SwissProt: P51878 Human)
• Localization: NLRP1 inflammasome complex, cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CASP5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:500-1:1000
• Control:

  Jurkat Staurosporine Treated / Untreated Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Caspase 5 Antibody (SD203-2)

• CASP5
• CASP-5
• caspase 5, apoptosis-related cysteine peptidase
• caspase 5, apoptosis-related cysteine protease
• Caspase5
• Caspase-5
• EC 3.4.22
• EC 3.4.22.58
• ICE(rel)III
• ICE(rel)-III
• ICEREL-III
• ICH3
• ICH-3
• MGC141966
• Protease ICH-3
• Protease TY
• TY protease

Background

Caspases are a family of cytosolic aspartate-specific cysteine proteases involved in the initiation and execution of apoptosis. Caspase-5 (ICH-3, ICEREL-III, TY protease) is a member of the caspase-1 subfamily and it is an upstream mediator of apoptosis. Caspase-5 is highly expressed in lung, liver, and skeletal muscle, and similar to Caspase-4, its overexpression can induce cellular apoptosis (1). A tetramer, Caspase-5 separates into two subunits of a 20kda (p20) and 10kda (p10) after activation. In vitro, Caspase-5 can be cleaved by GranB, a major player in Cytotoxic T lymphocytes (CTL) induced apoptosis (2). In vivo, the Caspase-5 expression is elevated by LPS and IFNg. Caspase-5 induced apoptosis can be inhibited by CrmA and Bcl-2. Caspase-5 has also been implicated in cellular inflammatory response and in the microsatelite mutator pathway for cancer (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CASP5