Caspase-4 Activity Assay Kit (Colorimetric)

• Reactivity: All-NA
• Applications: Flow
• Suitable Sample Type: Cell and tissue samples

Caspase-4 Activity Assay Kit (Colorimetric) Summary

• Description: Caspase 4, also known as Ich-2(ICE and CED-3 homolog 2), also known as TX and ICErel-II, can interact with Apaf-1 and participate in caspase 3 activation caused by cytochrome c. Caspase 4 can also interact with caspase 14. This caspase 4 activity assay kit is based on caspase 4 that can catalyze the substrate Ac-LEVD-pNA to generate yellow pNA (p-Nitroaniline). The pNA has a strong absorption near 405 nm, The activity of caspase 4 can be calculated by determining the absorbance at 405 nm.
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: CASP4

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
• Application Notes: Detection Instrument: Spectrophotometer Assay Time: 4-6 hours

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase-4 Activity Assay Kit (Colorimetric)

• apoptotic cysteine protease Mih1/TX
• CASP4
• CASP-4
• caspase 4, apoptosis-related cysteine peptidase
• caspase 4, apoptosis-related cysteine protease
• Caspase4
• Caspase-4
• EC 3.4.22
• EC 3.4.22.57
• ICE(rel)II
• ICE(rel)-II
• ICEREL-II
• ICH2
• ICH-2
• Mih1/TX
• Protease ICH-2
• Protease TX
• TX

Background

Caspase 4 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CASP4