Caspase-4 Activity Assay Kit (Colorimetric)

Product Discontinued

Caspase-4 Activity Assay Kit (Colorimetric) Summary

• Description: Simple and convenient colorimetric assay to measure Caspase-4 activity within 1-2 hrs.
  
  Detection method: Absorbance at 400 or 405 nm.Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65659)
• Kit Type: Activity Assay Kit (Colorimetric)
• Gene: CASP4

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase-4 Activity Assay Kit (Colorimetric)

• apoptotic cysteine protease Mih1/TX
• CASP4
• CASP-4
• caspase 4, apoptosis-related cysteine peptidase
• caspase 4, apoptosis-related cysteine protease
• Caspase4
• Caspase-4
• EC 3.4.22
• EC 3.4.22.57
• ICE(rel)II
• ICE(rel)-II
• ICEREL-II
• ICH2
• ICH-2
• Mih1/TX
• Protease ICH-2
• Protease TX
• TX

Background

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400 or 405 nm.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CASP4