Caspase-4 Colorimetric Assay Kit

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Product Details

Summary
Reactivity Hu, Mu, Rt, MaSpecies Glossary
Suitable Sample Type
Cell and tissue lysates
Kit Type
Colorimetric Assay Kit

Order Details

Caspase-4 Colorimetric Assay Kit Summary

Description
Caspase-4 Activity Colorimetric Assay Kit: Simple & Convenient Colorimetric Assay to Measure Caspase-4 Activity within 1-2 hrs. Kit Size: 25, 100, 200 and 400 assays.

  • Detection method- Fluorescence (Ex/Em 400/505)
  • Applications: Detects alternative proteolytic enzymes such as the lysosomal cathepsin proteases that can initiate or propagate proapoptotic signals.
Kit Type
Colorimetric Assay Kit
Gene
CASP4

Applications/Dilutions

Application Notes
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Colorimetric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate LEVD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm

Packaging, Storage & Formulations

Storage
Store at -20C.

Kit Components

Components
  1. 2X Reaction Buffer
  2. LEVD-pNA (4 mM)
  3. DTT (1 M)
  4. Dilution Buffer
  5. Cell Lysis Buffer

Alternate Names for Caspase-4 Colorimetric Assay Kit

  • apoptotic cysteine protease Mih1/TX
  • CASP4
  • CASP-4
  • caspase 4, apoptosis-related cysteine peptidase
  • caspase 4, apoptosis-related cysteine protease
  • Caspase4
  • Caspase-4
  • EC 3.4.22
  • EC 3.4.22.57
  • ICE(rel)II
  • ICE(rel)-II
  • ICEREL-II
  • ICH2
  • ICH-2
  • Mih1/TX
  • Protease ICH-2
  • Protease TX
  • TX

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Caspase-4 - a human protease with roles in inflammation and immunity
Caspases are a family of cysteine-aspartic acid proteases that cleave caspase proenzymes as well as other protein substrates. Caspases are well known for their role in apoptosis, but they also play a significant role in other cellular processes inc...  Read full blog post.

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Bioinformatics

Gene Symbol CASP4
Entrez