Caspase-4 Assay Kit (Fluorometric)

Product Discontinued

Caspase-4 Assay Kit (Fluorometric) Summary

• Description: Simple and convenient fluorometric assay to measure Caspase-4 activity within 1-2 hrs.
  
  
  Detection method: Fluorescence (Ex/Em = 400/505)Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
  
  
  This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab65658)
• Kit Type: Assay Kit (Fluorometric)
• Gene: CASP4

Packaging, Storage & Formulations

• Storage: Store at -20C.

Alternate Names for Caspase-4 Assay Kit (Fluorometric)

• apoptotic cysteine protease Mih1/TX
• CASP4
• CASP-4
• caspase 4, apoptosis-related cysteine peptidase
• caspase 4, apoptosis-related cysteine protease
• Caspase4
• Caspase-4
• EC 3.4.22
• EC 3.4.22.57
• ICE(rel)II
• ICE(rel)-II
• ICEREL-II
• ICH2
• ICH-2
• Mih1/TX
• Protease ICH-2
• Protease TX
• TX

Background

Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-4 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence LEVD. The assay is based on detection of cleavage of substrate LEVD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). LEVD-AFC emits blue light (400 nm); upon cleavage of the substrate by caspase-4 or related caspases, free AFC emits a yellow-green fluorescence (505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CASP4