Mouse Caspase-4 ELISA Kit (Colorimetric)

ELISA: Mouse Caspase-4 ELISA Kit (Colorimetric) [NBP2-75026] - Samples were spiked with high concentrations of Mouse Caspase-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse Caspase-4 ELISA Kit (Colorimetric) [NBP2-75026] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL

Mouse Caspase-4 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse CASP4 in samples. No significant cross-reactivity or interference between Mouse CASP4 and analogues was observed.
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.46%
• Intra-Assay: CV% < 5.05%
• Spike Recovery: 85-108%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CASP4

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse Caspase-4 ELISA Kit (Colorimetric)

• apoptotic cysteine protease Mih1/TX
• CASP4
• CASP-4
• caspase 4, apoptosis-related cysteine peptidase
• caspase 4, apoptosis-related cysteine protease
• Caspase4
• Caspase-4
• EC 3.4.22
• EC 3.4.22.57
• ICE(rel)II
• ICE(rel)-II
• ICEREL-II
• ICH2
• ICH-2
• Mih1/TX
• Protease ICH-2
• Protease TX
• TX

Background

Caspase 4 encodes a protein that is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a prodomain and a large and small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. This caspase is able to cleave and activate its own precursor protein, as well as caspase 1 precursor. When overexpressed, this gene induces cell apoptosis. Alternative splicing results in transcript variants encoding distinct isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for Caspase-4 ELISA Kit (NBP2-75026). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CASP4