Caspase-3 Antibody (84803) [Unconjugated]

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for 3 hours. PVDF membrane was probed with 1 µg/mL of Human Caspase‑3 Monoclonal Antibody (Catalog # MAB707), followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for precursor Caspase‑3 and the p18 subunit at approximately 36 and 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Caspase-3 knockout HeLa cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caspase‑3 Monoclonal Antibody (Catalog # MAB707) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Caspase‑3 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, KO
• Clone: 84803
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Caspase-3 Antibody (84803) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Caspase-3 (18 kDa subunit)
  aa 29-175
  Accession # P42574
• Specificity: Detects human precursor Caspase-3 and the 18 kDa subunit generated during apoptosis.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CASP3

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Knockout Validated
• Control:

  Caspase-3 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-3 Antibody (84803) [Unconjugated]

• Apopain
• apoptosis-related cysteine protease
• CASP3
• CASP-3
• caspase 3, apoptosis-related cysteine peptidase
• Caspase3
• Caspase-3
• CPP32
• CPP-32
• CPP32B
• CPP32SREBP cleavage activity 1
• Cysteine protease CPP32
• EC 3.4.22
• EC 3.4.22.56
• LICE-1
• PARP cleavage protease
• procaspase3
• Protein Yama
• SCA-1
• YAMA

Background

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed, and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

 

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-3 Antibody (MAB707)(6)

Publications using MAB707

• Sharp A, Heazell A, Baczyk D, Dunk C, Lacey H, Jones C, Perkins J, Kingdom J, Baker P, Crocker I Preeclampsia is associated with alterations in the p53-pathway in villous trophoblast. PLoS ONE, 2014;9(1):e87621. 2014 [PMID: 24498154] (Western Blot, Human)
• Seo K, Kim J, Park J, Song K, Yun E, Park J, Kweon G, Yoon W, Lim K, Hwang B PMA synergistically enhances apicularen A-induced cytotoxicity by disrupting microtubule networks in HeLa cells. BMC Cancer, 2014;14(0):36. 2014 [PMID: 24447339] (Western Blot, Human)
• Ghosh JG, Shenoy AK, Clark JI Interactions between important regulatory proteins and human alphaB crystallin. Biochemistry, 2007;46(21):6308-17. 2007 [PMID: 17487982] (ELISA-based Protein Pin Array, Human)
• Choo-Wing R, Nedrelow JH, Homer RJ, Elias JA, Bhandari V Developmental differences in the responses of IL-6 and IL-13 transgenic mice exposed to hyperoxia. Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L142-50. 2007 [PMID: 17400600] (IHC-P, Mouse)
• Vallon M, Essler M Proteolytically processed soluble tumor endothelial marker (TEM) 5 mediates endothelial cell survival during angiogenesis by linking integrin alpha(v)beta3 to glycosaminoglycans. J. Biol. Chem., 2006;281(45):34179-88. 2006 [PMID: 16982628] (Western Blot, Human)
• You RI, Chou YC Inhibition of lymphotoxin-beta receptor-mediated cell death by survivin-DeltaEx3. Cancer Res., 2006;66(6):3051-61. 2006 [PMID: 16540654] (Immunoprecipitation, Human)

Bioinformatics

• Gene Symbol: CASP3
• Uniprot:
  • P42574 ()