Caspase-3 Antibody (269518) [Unconjugated] - Cleaved (Asp175)

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Cleaved Caspase‑3 (Asp175) Monoclonal Antibody (MAB835), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Cleaved Caspase‑3 (Asp175) at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Caspase‑3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with staurosporin using Human/Mouse Cleaved Caspase‑3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Jurkat human acute T cell leukemia cell line untreated (open histogram) or treated with 3 μM Staurosporine for 3 hours (filled histogram) was stained with Rabbit Anti-Human/Mouse Caspase‑3 Monoclonal Antibody (Catalog # MAB835, filled histogram) followed by anti-Rabbit IgG FITC-conjugated secondary antibody (Catalog # F0112). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with 90% methanol. View our protocol for Staining Intracellular Molecules.

Caspase‑3 was detected in immersion fixed paraffin-embedded sections of human colon cancer tissue using Rabbit Anti-Human/Mouse Cleaved Caspase‑3 (Asp175) Monoclonal Antibody (Catalog # MAB835) at 0.3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu, Mu
• Applications: WB, IHC, Flow, ICC/IF
• Clone: 269518
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

Caspase-3 Antibody (269518) [Unconjugated] - Cleaved (Asp175) Summary

• Immunogen: KLH-conjugated human Caspase-3 synthetic peptide
  CRGTELDCGIETD
  Accession # U26943
• Specificity: Detects human and mouse Caspase-3 cleaved at Asp175. No cross-reactivity was detected with the full-length procaspase-3 or other caspases.
• Source: N/A
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CASP3

Applications/Dilutions

• Dilutions:
  • Western Blot 0.5 ug/mL
  • Immunohistochemistry 0.3-25 ug/mL
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-3 Antibody (269518) [Unconjugated] - Cleaved (Asp175)

• Apopain
• apoptosis-related cysteine protease
• CASP3
• CASP-3
• caspase 3, apoptosis-related cysteine peptidase
• Caspase3
• Caspase-3
• CPP32
• CPP-32
• CPP32B
• CPP32SREBP cleavage activity 1
• Cysteine protease CPP32
• EC 3.4.22
• EC 3.4.22.56
• LICE-1
• PARP cleavage protease
• procaspase3
• Protein Yama
• SCA-1
• YAMA

Background

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Caspase-3 Antibody (MAB835)(43)

Publications using MAB835

• S Giraud, C Steichen, P Couturier, S Tillet, V Mallet, R Coudroy, JM Goujon, P Hannaert, T Hauet Influence of Hypoxic Preservation Temperature on Endothelial Cells and Kidney Integrity Biomed Res Int, 2019;2019(0):8572138. 2019 [PMID: 31275986] (IHC-P, Porcine)
• KR Greenow, M Zverev, S May, H Kendrick, GT Williams, T Phesse, L Parry Lect2 deficiency is characterised by altered cytokine levels and promotion of intestinal tumourigenesis Oncotarget, 2018;9(92):36430-36443. 2018 [PMID: 30559928] (IHC, Mouse)
• H Zahednasab, M Firouzi, S Kaboudania, Z Mojallal-T, S Karampour, H Keyvani The protective effect of rifampicin on behavioral deficits, biochemical, and neuropathological changes in a cuprizone model of demyelination Cytokine, 2019;113(0):417-426. 2019 [PMID: 30539784] (Western Blot, Mouse)
• D Liu, T Li, H Luo, X Zuo, S Liu, S Wu The effect of the cholinergic anti-inflammatory pathway on collagen-induced arthritis involves the modulation of dendritic cell differentiation Arthritis Res. Ther., 2018;20(1):263. 2018 [PMID: 30486874] (Western Blot, Mouse)
• ZP You, MJ Yu, YL Zhang, K Shi Progranulin protects the mouse retina under hypoxic conditions via inhibition of the Toll?like receptor?4?NADPH oxidase 4 signaling pathway Mol Med Rep, 2018;0(0):. 2018 [PMID: 30431085] (Western Blot, Mouse)
• T Rahim, P Becquart, ME Baeva, J Quandt Expression of the neuroprotective protein aryl hydrocarbon receptor nuclear translocator 2 correlates with neuronal stress and disability in models of multiple sclerosis J Neuroinflammation, 2018;15(1):270. 2018 [PMID: 30231889] (ICC, Rat)
• R Hamon, HB Tran, E Roscioli, M Ween, H Jersmann, S Hodge Bushfire smoke is pro-inflammatory and suppresses macrophage phagocytic function Sci Rep, 2018;8(1):13424. 2018 [PMID: 30194323] (ICC, Human)
• P Shen, J Chen, M Pan The protective effects of total paeony glycoside on ischemia/reperfusion injury in H9C2 cells via inhibition of the PI3K/Akt signaling pathway Mol Med Rep, 2018;18(3):3332-3340. 2018 [PMID: 30066927] (Western Blot, Rat)
• LG Que, Z Yang, NL Lugogo, RK Katial, SA Shoemaker, JM Troha, DM Rodman, RM Tighe, M Kraft Effect of the S-nitrosoglutathione reductase inhibitor N6022 on bronchial hyperreactivity in asthma Immun Inflamm Dis, 2018;0(0):. 2018 [PMID: 29642282] (Western Blot, Mouse)
• RL Rausch, RT Libby, AE Kiernan Trabecular meshwork morphogenesis: A comparative analysis of wildtype and anterior segment dysgenesis mouse models Exp. Eye Res., 2018;0(0):. 2018 [PMID: 29452107] (IHC-Fr, Mouse)
• BS Moon, J Bai, M Cai, C Liu, J Shi, W Lu Kruppel-like factor 4-dependent Staufen1-mediated mRNA decay regulates cortical neurogenesis Nat Commun, 2018;9(1):401. 2018 [PMID: 29374155] (IHC, Mouse)
• A Shaalan, G Carpenter, G Proctor Caspases are key regulators of inflammatory and innate immune responses mediated by TLR3 in vivo Mol. Immunol., 2018;94(0):190-199. 2018 [PMID: 29331803] (IHC-P, Mouse)
• L Zhang, Y Yang, S Li, S Zhang, X Zhu, Z Tai, M Yang, Y Liu, X Guo, B Chen, Z Jiang, F Lu, X Zhu Loss of Tmem30a leads to photoreceptor degeneration Sci Rep, 2017;7(1):9296. 2017 [PMID: 28839191] (IHC, Mouse)
• H Zhang, H Yan, X Zhou, H Wang, Y Yang, J Zhang, H Wang The protective effects of Resveratrol against radiation-induced intestinal injury BMC Complement Altern Med, 2017;17(1):410. 2017 [PMID: 28814292] (IHC-P, Mouse)
• F Sallustio, C Curci, A Aloisi, CC Toma, E Marulli, G Serino, SN Cox, G De Palma, A Stasi, C Divella, R Rinaldi, FP Schena Inhibin-A and Decorin Secreted by Human Adult Renal Stem/Progenitor Cells Through the TLR2 Engagement Induce Renal Tubular Cell Regeneration Sci Rep, 2017;7(1):8225. 2017 [PMID: 28811645] (Flow Cytometry, Human)
• S Janczar, J Nautiyal, Y Xiao, E Curry, M Sun, E Zanini, AJ Paige, H Gabra WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response Cell Death Dis, 2017;8(7):e2955. 2017 [PMID: 28749468] (Western Blot, Human)
• N Prevete, F Liotti, A Illiano, A Amoresano, P Pucci, A de Paulis, RM Melillo Formyl peptide receptor 1 suppresses gastric cancer angiogenesis and growth by exploiting inflammation resolution pathways Oncoimmunology, 2017;6(4):e1293213. 2017 [PMID: 28507800] (IHC-P, Human)
• K Sato, R Shirai, M Hontani, R Shinooka, A Hasegawa, T Kichise, T Yamashita, H Yoshizawa, R Watanabe, TA Matsuyama, H Ishibashi-, S Koba, Y Kobayashi, T Hirano, T Watanabe Potent Vasoconstrictor Kisspeptin-10 Induces Atherosclerotic Plaque Progression and Instability: Reversal by its Receptor GPR54 Antagonist J Am Heart Assoc, 2017;6(4):. 2017 [PMID: 28411243] (Western Blot, Human)
• C Andreani, C Bartolacci, K Wijnant, R Crinelli, M Bianchi, M Magnani, A Hysi, M Iezzi, A Amici, C Marchini Resveratrol fuels HER2 and ER?-positive breast cancer behaving as proteasome inhibitor Aging (Albany NY), 2017;9(2):508-523. 2017 [PMID: 28238967] (IHC, Mouse)
• X Wang, GX Xue, WC Liu, H Shu, M Wang, Y Sun, X Liu, YE Sun, CF Liu, J Liu, W Liu, X Jin Melatonin alleviates lipopolysaccharide-compromised integrity of blood-brain barrier through activating AMP-activated protein kinase in old mice Aging Cell, 2017;0(0):. 2017 [PMID: 28156052] (IHC-Fr, Mouse)
• Lucian Beer Ionizing radiation regulates long non-coding RNAs in human peripheral blood mononuclear cells J. Radiat. Res, 2016;0(0):. 2016 [PMID: 27974506] (Western Blot, Human)
• H-Ras and K-Ras oncoproteins induce different tumor spectra when driven by the same regulatory sequences Cancer Res., 2016;0(0):. 2016 [PMID: 27872088] (IHC-P, Mouse)
• Elaine Fuchs Impaired Epidermal to Dendritic T Cell Signaling Slows Wound Repair in Aged Skin Cell, 2016;167(5):1323-1338.e14. 2016 [PMID: 27863246] (IHC, Mouse)
• Chimeric Mice with Competent Hematopoietic Immunity Reproduce Key Features of Severe Lassa Fever PLoS Pathog, 2016;12(5):e1005656. 2016 [PMID: 27191716] (IHC-P, Mouse)
• Tania L Slatter Antitumor cytotoxicity induced by bone-marrow-derived antigen-presenting cells is facilitated by the tumor suppressor protein p53 via regulation of IL-12 Oncoimmunology, 2016;5(3):e1112941. 2016 [PMID: 27141366] (IHC, Mouse)
• Himmelein S, Lindemann A, Sinicina I, Strupp M, Brandt T, Hufner K Latent herpes simplex virus 1 infection does not induce apoptosis in human trigeminal Ganglia. J Virol, 2015;89(10):5747-50. 2015 [PMID: 25762734] (IHC-Fr, Human)
• Prevete N, Liotti F, Visciano C, Marone G, Melillo R, de Paulis A The formyl peptide receptor 1 exerts a tumor suppressor function in human gastric cancer by inhibiting angiogenesis. Oncogene, 2015;34(29):3826-38. 2015 [PMID: 25263443] (IHC-Fr, Mouse)
• Diefenbacher M, Popov N, Blake S, Schulein-Volk C, Nye E, Spencer-Dene B, Jaenicke L, Eilers M, Behrens A The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer. J Clin Invest, 2014;124(8):3407-18. 2014 [PMID: 24960159] (IHC, Mouse)
• Yuan, Dongqing, Xu, Yidan, Hang, Hui, Liu, Xiaoyi, Chen, Xi, Xie, Ping, Yuan, Songtao, Zhang, Weiwei, Lin, Xiaojun, Liu, Qinghuai Edaravone protect against retinal damage in streptozotocin-induced diabetic mice. PLoS ONE, 2014;9(6):e99219. 2014 [PMID: 24897298] (Western Blot, Mouse)
• Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta. Proc Natl Acad Sci U S A, 2014;111(21):E2229-36. 2014 [PMID: 24825889] (Western Blot, Human)
• Oestereich L, Rieger T, Neumann M, Bernreuther C, Lehmann M, Krasemann S, Wurr S, Emmerich P, de Lamballerie X, Olschlager S, Gunther S Evaluation of antiviral efficacy of ribavirin, arbidol, and T-705 (favipiravir) in a mouse model for Crimean-Congo hemorrhagic fever. PLoS Negl Trop Dis, 2014;8(5):e2804. 2014 [PMID: 24786461] (IHC, Mouse)
• Liu L, Salnikov A, Bauer N, Aleksandrowicz E, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Schemmer P, Werner J, Herr I Triptolide reverses hypoxia-induced epithelial-mesenchymal transition and stem-like features in pancreatic cancer by NF-kappaB downregulation. Int J Cancer, 2014;134(10):2489-503. 2014 [PMID: 24615157] (IHC-Fr, Human)
• Hahn W, Kuzmicic J, Burrill J, Donoghue M, Foncea R, Jensen M, Lavandero S, Arriaga E, Bernlohr D Proinflammatory cytokines differentially regulate adipocyte mitochondrial metabolism, oxidative stress, and dynamics. Am J Physiol Endocrinol Metab, 2014;306(9):E1033-45. 2014 [PMID: 24595304] (Western Blot, Mouse)
• Hage C, Rausch V, Giese N, Giese T, Schonsiegel F, Labsch S, Nwaeburu C, Mattern J, Gladkich J, Herr I The novel c-Met inhibitor cabozantinib overcomes gemcitabine resistance and stem cell signaling in pancreatic cancer. Cell Death Dis, 2013;4(0):e627. 2013 [PMID: 23661005] (IHC, Human)
• Janek T, Krasowska A, Radwanska A, Lukaszewicz M Lipopeptide biosurfactant pseudofactin II induced apoptosis of melanoma A 375 cells by specific interaction with the plasma membrane. PLoS ONE, 2013;8(3):e57991. 2013 [PMID: 23483962] (IHC, Human)
• Downs I, Liu J, Aw TY, Adegboyega PA, Ajuebor MN The ROS scavenger, NAC, regulates hepatic Vα14iNKT cells signaling during Fas mAb-dependent fulminant liver failure. PLoS ONE, 2012;7(6):e38051. 2012 [PMID: 22701598] (Western Blot, Mouse)
• Goldie S, Mulder K, Tan D, Lyons S, Sims A, Watt F FRMD4A upregulation in human squamous cell carcinoma promotes tumor growth and metastasis and is associated with poor prognosis. Cancer Res, 2012;72(13):3424-36. 2012 [PMID: 22564525] (IHC-Fr, Human)
• Chen RY, Chen HX, Lin JX In-vivo transfection of pcDNA3.1-IGFBP7 inhibits melanoma growth in mice through apoptosis induction and VEGF downexpression. J. Exp. Clin. Cancer Res., 2010;29(0):13. 2010 [PMID: 20158915] (IHC-Fr, Mouse)
• Han Y, Wu G, Deng J, Tao J, Guo L, Tian X, Kang J, Zhang X, Yan C Cellular repressor of E1A-stimulated genes inhibits human vascular smooth muscle cell apoptosis via blocking P38/JNK MAP kinase activation. J. Mol. Cell. Cardiol., 2010;48(6):1225-35. 2010 [PMID: 20060003] (Western Blot, Human)
• Deng J, Han Y, Yan C, Tian X, Tao J, Kang J, Li S Overexpressing cellular repressor of E1A-stimulated genes protects mesenchymal stem cells against hypoxia- and serum deprivation-induced apoptosis by activation of PI3K/Akt. Apoptosis, 2010;15(4):463-73. 2010 [PMID: 19997978] (Western Blot, Rat)
• Terayama R, Bando Y, Murakami K, Kato K, Kishibe M, Yoshida S Neuropsin promotes oligodendrocyte death, demyelination and axonal degeneration after spinal cord injury. Neuroscience, 2007;148(1):175-87. 2007 [PMID: 17629414] (Western Blot, Mouse)
• Olivotto E, Vitellozzi R, Fernandez P, Falcieri E, Battistelli M, Burattini S, Flamigni F, Santi S, Borzi RM Chondrocyte hypertrophy and apoptosis induced by GROalpha require three-dimensional interaction with the extracellular matrix and a co-receptor role of chondroitin sulfate and are associated with the mitochondrial splicing variant of cathepsin B. J. Cell. Physiol., 2007;210(2):417-27. 2007 [PMID: 17096385] (IHC, Human)
• Raz V, Carlotti F, Vermolen BJ, van der Poel E, Sloos WC, Knaan-Shanzer S, de Vries AA, Hoeben RC, Young IT, Tanke HJ, Garini Y, Dirks RW Changes in lamina structure are followed by spatial reorganization of heterochromatic regions in caspase-8-activated human mesenchymal stem cells. J. Cell. Sci., 2006;119(0):4247-56. 2006 [PMID: 17003109] (ICC, Human)

Reviews for Caspase-3 Antibody (MAB835) (4)

Average Rating: 4.5

(Based on 4 reviews)

We have 4 reviews tested in 2 species: Human, Mouse.

reviewed by: Anonymous WB Human 02/25/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 22160308
• Species: Human

Blocking

• Blocking Details: See PMID 22160308

Details

• Detection Notes: See PMID 22160308

Comments

• Comments: Independent review from antibodyresource.comThe antibody is very specific and the blot is clean with only the specific bands in the correct molecular weight (published in PMID 22160308).

reviewed by: Anonymous IF Human 02/25/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 23483962
• Species: Human

Blocking

• Blocking Details: See PMID 23483962

Details

• Detection Notes: See PMID 23483962

Comments

• Comments: Independent review from antibodyresource.comPublished in PMID 23483962

reviewed by: Anonymous WB Human 02/25/2015

Summary

• Application: Western Blot
• Sample Tested: See PMID 23536519
• Species: Human

Blocking

• Blocking Details: See PMID 23536519

Details

• Detection Notes: See PMID 23536519

Comments

• Comments: Independent review from antibodyresource.com

reviewed by: Anonymous IHC-P Mouse 02/25/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 23637762
• Species: Mouse
• Special Applications: Independent review from antibodyresource.comPublished in PMID 23637762

Bioinformatics

• Gene Symbol: CASP3
• Uniprot:
  • U26943()