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Caspase-3 Antibody (1112I) [Unconjugated]

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Caspase-3 mRNA was detected (top image) informalin-fixed paraffin-embedded tissue sections of human colon probed with ACDRNAScope® Probe (Catalog # 436561) and stained using ACD RNAscope® 2.5 HDDuplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section (bottom image) wasprocessed for immunohistochemistry using R&D Systems Rabbit Anti-HumanCaspase-3 Monoclonal Antibody (Catalog# MAB7071) at 5 ug/mL for 1 hour at roomtemperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP PolymerAntibody (R&D Systems, Catalog # VC001) and DAB chromogen (brown). Tissueswere counterstained with hematoxylin (blue).
Caspase-3 mRNA was detected (top image) informalin-fixed paraffin-embedded tissue sections of human colon probed with ACDRNAScope® Probe (Catalog # 436561) and stained using ACD RNAscope® 2.5 HDDuplex ...read more
Caspase‑3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with Staurosporine using Rabbit Anti-Human Caspase‑3 Monoclonal Antibody (Catalog # MAB7071) at 1 µg/mL for 3 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Dual ISH-IHC, ICC/IF
Clone
1112I
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7071-SP
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Caspase-3 Antibody (1112I) [Unconjugated] Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Human Caspase-3 synthetic peptide
Accession # P42574
Specificity
Detects human Caspase-3 in direct ELISAs.
Source
N/A
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
CASP3
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Dxxxxxxxxxxxxxxx 5-25 ug/mL
  • Immunocytochemistry 1-25 ug/mL
Control
Caspase-3 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
Sodium Azide
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Caspase-3 Antibody (1112I) [Unconjugated]

  • Apopain
  • apoptosis-related cysteine protease
  • CASP3
  • CASP-3
  • caspase 3, apoptosis-related cysteine peptidase
  • Caspase3
  • Caspase-3
  • CPP32
  • CPP-32
  • CPP32B
  • CPP32SREBP cleavage activity 1
  • Cysteine protease CPP32
  • EC 3.4.22
  • EC 3.4.22.56
  • LICE-1
  • PARP cleavage protease
  • procaspase3
  • Protein Yama
  • SCA-1
  • YAMA

Background

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme. There is one potential variant that shows an alternative start site nine aa upstream of the standard start site coupled with a 21 aa substitution for aa 162-277. Over aa 29-175, human and mouse caspase-3 share 87% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Control Lysate(s)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CASP3
Uniprot