Caspase-14 Antibody

Images

 
Western Blot: Caspase-14 Antibody [NB100-56126] - Analysis of Caspase-14. Tissue lysates (50 ug/lane) and recombinant human Caspase-14 were (Hu C14, 15 ng) were western blotted with Caspase-14 antibody at 1:2000. The ...read more
Immunohistochemistry: Caspase-14 Antibody [NB100-56126] - Immunohistochemistry-Paraffin: Caspase-14 Antibody [NB100-56126] - Tissue sections of mouse skin at E17 stained using this antibody at 1:500.
Immunohistochemistry-Paraffin: Caspase-14 Antibody [NB100-56126] - Formalin-fixed paraffin-embedded sections from a human ovarian cancer tissue microarray stained for Caspase-14 expression using this antibody at ...read more
Immunohistochemistry-Paraffin: Caspase-14 Antibody [NB100-56126] - Tissue sections of human cervix stained using this antibody at 1:2000. A. Normal cervix (squamous epithelium). B. CIN1 (low-grade squamous ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Caspase-14 Antibody Summary

Immunogen
Recombinant full-length human Caspase-14 was used as immunogen.
Specificity
This polyclonal antibody recognizes the proform of caspase-14 (~-28-32 kDa), and the large (~14-21 kDa) and small (~10-11 kDa) of active/cleaved caspase-14.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CASP14
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
Positive Control
Brain Lysate (NB820-59177)
Publications
Read Publications using
NB100-56126 in the following applications:

  • IHC
    3 publications
  • 1 publication
  • WB
    1 publication

Reactivity Notes

Cross reacts with Dog, Gerbil, Human, Mouse and Rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Neat whole antisera
Preservative
0.02% Sodium Azide
Purity
Unpurified

Alternate Names for Caspase-14 Antibody

  • apoptosis-related cysteine protease
  • CASP14
  • CASP-14
  • caspase 14, apoptosis-related cysteine peptidase
  • caspase 14, apoptosis-related cysteine protease
  • Caspase14
  • Caspase-14
  • EC 3.4.22.-
  • MGC119078
  • MGC119079
  • MICE

Background

Apoptosis, or programmed cell death, is a common property of all multicellular organisms. The current dogma of apoptosis suggests that the components of the core cell-death machinery are integral to cells and widely conserved across species. Caspases, a family of cysteinyl aspartate-specific proteases, are integral components of the cell death machinery (reviewed in Siegal, 2006; and Lavrik et al, 2005). They play a central role in the initiation and execution of apoptotic cell death and in inflammation. Caspases are typically divided into 3 major groups, depending on the structure of their prodomain and their function. Group 1: inflammatory caspases (caspases 1, 4, 5, 11, 12, 14). Group II: initiator of apoptosis caspases (caspases 2, 8, 9). Group II: effector caspases (caspases 3, 6, 7). Caspases are constitutively expressed in almost all cell types as inactive proenzymes (zymogens: enzyme precursors which require a biochemical change to become active enzymes) that are processed and activated in response to a variety of pro-apoptotic or inflammatory stimuli. The procaspases (32-56 kDa) contain four domains: an N-terminal prodomain (2-25 kDa), a large subunit (p20: 17-21 kDa), a small subunit (p10: 10-13 kDa) and a short linker region between the large and small subunits. Caspase activation involves proteolytic processing of the proenzyme at specific aspartate residues between the domains. This results in removal of the prodomain as well as the linker region and formation of a heterodimer containing one large and one small subunit (p20-p10). The active caspase is a tetramer composed of two heterodimers (p202-p102). Active caspases mediate cell death and inflammation through cleavage of particular cellular substrates that are involved in these processes. Caspase-14 activation has been implicated in keratinocyte senescence which leads to the cornified cell layer, suggesting a role for caspase-14 in epithelial cell differentiation (Pistritto et al. 2002). Terminal differentiation of keratinocytes has some features of classical apoptosis including DNA fragmentation, nuclear condensation, and induction of cross-linking transglutaminases. Tumor-specific alterations in caspase-14 expression have been found in epithelial malignancies, suggesting that caspase-14 plays a role in epithelial cell transformation. The caspase-14 NB100-56126 antibody recognizes the proform of caspase-14 (approx. 28-32 kDa), and the large (approx. 14-21 kDa) and small (approx. 10-11 kDa) of active/cleaved caspase-14.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for Caspase-14 Antibody (NB100-56126)(5)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(3)
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
Rat
(2)
All Species
Showing Publications 1 - 5 of 5.
Publications using NB100-56126 Applications Species
Chamcheu JC, Adhami VM, Esnault S et al. Dual inhibition of PI3K/Akt and mTOR by the Dietary Antioxidant Delphinidin Ameliorates Psoriatic Features In-vitro and in an Imiquimod-induced Psoriasis-like Disease in Mice Antioxid. Redox Signal. 2016 Jul 08 [PMID: 27393705] (IHC, Human) IHC Human
Murakami H, Okamura K, Aoki S et al. Association of caspase-14 and filaggrin expression with keratinization of the oral mucosa and reconstruction culture rat models. J Periodontal Res 2013 Dec 11 [PMID: 24329962] (IHC, Rat) IHC Rat
Krajewska M, Kim H, Shin E et al. Tumor-associated alterations in caspase-14 expression in epithelial malignancies. Clin Cancer Res. 2005 Aug 1 [PMID: 16061862] (WB, IHC-P)

Details:
WB: Fig 1C (recombinant human and mouse caspase-14 protein) and Fig 1D (various normal human tissues and tumor cell lines); IHC (paraffin), Fig 2 (continuum from human uterine normal to malignant cervix), Fig 3A (human gastric cancer), Fig 4 (human ovaria
WB, IHC-P
Krajewska M, Rosenthal RE, Mikolajczyk J et al. Early processing of Bid and caspase-6, -8, -10, -14 in the canine brain during cardiac arrest and resuscitation. Exp Neurol. 2004 Oct [PMID: 15380478]

Details:
Antibodies cited: 1. Caspase-9 (Active/Cleaved), IMG-5705: WB: Fig 1A (recombinant human caspase-9), Fig 4A (recombinant human caspase-9, rat brain), Fig 4B-F (isolated mitochondria from rat liver or heart). Fig 5 (PC12 cells) IHC (P): Fig 2d-k (rat kidne
Arai M, Matsuzaki T, Ihara S. Wound Closure on the Neonatal Rat Skin I. The Modulation of the Thickness of Epidermis at the Closing Incisional Wounds. CellBio 2013 (IHC, Rat) IHC Rat

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Positive Control Lysate(s)

Secondary Antibodies

 

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Blogs on Caspase-14.

Caspase 14 - A unique caspase needed for skin differentiation
Caspases are typically known for their role in cell death. However some caspases have recently been investigated for their function during cell proliferation and differentiation. Of these caspase-14 shows a unique expression pattern in the skin and...  Read full blog post.

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Bioinformatics

Gene Symbol CASP14
Entrez
Uniprot