CASK Antibody - BSA Free

Western Blot: CASK Antibody [NBP2-41181] - Western blot analysis of CASK in mouse brain tissue lysate with CASK antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.

Simple Western: CASK Antibody [NBP2-41181] - 0.6 mg/ml protein lysates from differentiating human neuroepithelial stem cells run on Simple Western with 1:500 CASK.Ab (blue) and 1:1000 CASK.Ab (pink) and an actin antibody as positive control. The CASK peak is located at 116 kDa and actin at 38 kDa. Image is courtesy of customer.

Simple Western: CASK Antibody - BSA Free [NBP2-41181] - Presynaptic effect is limited to inhibitory VGAT presynaptic marker.a Deconvolution of cell-type populations underlying bulk RNA-sequence samples from all cell lines. b Quantification of VGlut & Homer-1 particle size (n = 4 per cell line). c Representative confocal microscopy images of neurons differentiated for 28 days & immunostained for inhibitory VGAT (green), Homer-1 (red), & Hoechst (blue). Scale = 10 µm. d Quantification of VGAT & Homer-1 particle size (n = 3 per cell line). Statistical differences between cell lines were calculated using ANOVA with post hoc Tukey HSD. *p < 0.05, **p < 0.01, ***p < 0.001. Asterisks are color-coded according to case cell lines. e In vivo concentration of GABA in the DLPFC, MFC, & putamen of ASDCASK_SS & his cotwin in relation to a typical developed control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32929080), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: CASK Antibody - BSA Free [NBP2-41181] - Immunofluorescence of CASK in human brain tissue with CASK antibody at 20 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

CASK Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CASK. The immunogen is located within amino acids 760 - 810 of CASK. Amino Acid Squence: YPIPHTTRPPKKDEENGKN
• Specificity: At least six alternatively spliced transcript variants have been observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CASK
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Simple Western
  • Western Blot 1 ug/mL
• Application Notes: Simple Western reported in a verified customer review.
  See Simple Western Antibody Database for Simple Western validation: Tested in Differentiating human neuroepithelial stem cells, separated by Size, antibody dilution of 1:500 and 1:1000, apparent MW was 116, 38 kDa

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for CASK Antibody - BSA Free

• calcium/calmodulin-dependent serine protein kinase (MAGUK family)
• calcium/calmodulin-dependent serine protein kinase membrane-associatedguanylate kinase
• Calcium/calmodulin-dependent serine protein kinase
• CAMGUK
• CMG
• EC 2.7.11
• EC 2.7.11.1
• FGS4CAGH39
• FLJ22219
• hCASK
• LIN2FLJ31914
• MICPCH
• peripheral plasma membrane protein CASK
• Protein lin-2 homolog
• TNRC8
• trinucleotide repeat containing 8

Background

Calmodulin sensitive kinase (CASK/mLIN-2) is a ~112-kDa member of the membrane associated quanylate kinase (MAGUK) protein family with multiple protein binding domains. These include an N-terminal calmodulin kinase (CaMKII) domain and C-terminal PDZ, SH3-, and quanylate kinase domains. CASK possesses both tyrosine kinase and serine/threonine kinase activity. In addition, CASK can form a heterotrimeric complex with Mint (X11or mLIN-10) and Veli (mLIN-7) proteins which is predicted to participate in plasma membrane receptor localization. CASK is localized to neuronal synapses and also interacts with syndecan and neurexin. The guanylate kinase domain of CASK has been shown to interact with the T-box transcription factor, Tbr-1, as a coactivator to induce transcription of genes that contain T-elements.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CASK Antibody (NBP2-41181)(2)

Publications using NBP2-41181

• Becker M, Mastropasqua F, Reising JP et al. Presynaptic dysfunction in CASK-related neurodevelopmental disorders Transl Psychiatry 2020-09-14 [PMID: 32929080] (ICC/IF, Simple Western, WB, Human)
• Li D Genetic implications of individual intervention and neuronal dysfunction in neurodevelopmental disorders Thesis 2022-01-01 (ICC/IF, WB, Human)

Review for CASK Antibody (NBP2-41181) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Martin Becker Simple Western Human 03/13/2017

Summary

• Application: Simple Western
• Sample Tested: Differentiated neuron progenitor cells
• Species: Human

Comments

• Comments: Protein lysates have been generated using detergent free lysis buffer (TrisHCl, NaCl, EDTA, EGTA) and 5 seconds of sonication.

Bioinformatics

• Gene Symbol: CASK