Carm1 Antibody

Images

 
Western Blot: Carm1 Antibody [NB200-341] - Detection of Human and Mouse CARM1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity ...read more
Immunohistochemistry: Carm1 Antibody [NB200-341] - Sample: FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-CARM1/PRMT4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB staining ...read more
Western Blot: Carm1 Antibody [NB200-341] - Whole HeLa cell lysate (25 mcg for WB in A; 5 mg for IP in B).antibody used at 0.1 mcg/ml for WB and at 4 mcg/5 mg lysate for IP. Detection: Chemiluminescence A. Coomassie ...read more
Immunohistochemistry-Paraffin: Carm1 Antibody [NB200-341] - Human stomach carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Carm1 Antibody Summary

Immunogen
A synthetic peptide that maps to a region between residues 450 and 500 of human Coactivator-Associated Arginine Methyltransferase 1 using the numbering given in Swiss-Prot entry Q86X55 (GeneID 10498).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CARM1
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1-4 ug/mg lysate
Application Notes
Optimal working dilutions should be determined experimentally by the investigator.Suggested starting dilutions are as follows. Western Blot: 1:5,000-1:15,000. *In some cases, the antibody may be diluted further than indicated.
Positive Control
HeLa Lysate (NB800-PC1)
Carm1 Lysate (NBL1-08698)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Carm1 Antibody

  • Carm1
  • coactivator-associated arginine methyltransferase 1EC 2.1.1.-
  • EC 2.1.1
  • histone-arginine methyltransferase CARM1
  • PRMT4
  • PRMT4EC 2.1.1.125
  • Protein arginine N-methyltransferase 4

Background

Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT4 belongs to a family of proteins that catalyzes the methylation of arginine residues. The PRMT proteins are recruited by C-terminal activation domain 2 of the SRC (steroid receptor coactivator) GRIP1, whereas p300 interacts with activation domain 1 in the C terminus. CARM1 can methylate histone H3 in vitro, and a mutation in the putative S-adenosylmethionine binding domain of CARM1 substantially reduces both methyltransferase and coactivator activities suggesting that coactivator mediated methylation of proteins in the transcription machinery may contribute to transcriptional regulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol CARM1
Entrez
Uniprot