CARHSP1 Antibody - Azide and BSA Free

Western Blot: CARHSP1 Antibody [NBP3-00026] - Anti-CARHSP1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 293T Hela Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 16 kDa. Observed band size: 22 kDa (We are unsure as to the identity of these extra bands).

Immunoprecipitation: CARHSP1 Antibody [NBP3-00026] - CARHSP1 was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate. Lane B: 0.5 mg 293T Whole Cell Lysate 2 ul anti-CARHSP1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-CARHSP1 rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 22 kDa. Observed band size: 22 kDa

• Reactivity: Hu
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CARHSP1 Antibody - Azide and BSA Free Summary

• Description: This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with purified, recombinant Human CARHSP1 (Uniprot#: Q9Y2V2; Met1-Ser147)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CARHSP1
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:10000
  • Immunoprecipitation 0.5-2 uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.2 um filtered solution in PBS
• Preservative: No Preservative
• Purity: Antigen and protein A Affinity-purified

Alternate Names for CARHSP1 Antibody - Azide and BSA Free

• calcium regulated heat stable protein 1 (24kD)
• calcium regulated heat stable protein 1, 24kDa
• calcium-regulated heat stable protein 1
• calcium-regulated heat-stable protein (24kD)
• Calcium-regulated heat-stable protein of 24 kDa
• CRHSP-24MGC111446
• CSDC1

Background

CARHSP1 may play a role in acinar cell metabolism

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CARHSP1