Cardiotrophin-1/CT-1 Antibody (91907) [Alexa Fluor® 532]

• Reactivity: Mu
• Applications: WB, ELISA(Cap), ELISA(Det)
• Clone: 91907
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

Cardiotrophin-1/CT-1 Antibody (91907) [Alexa Fluor® 532] Summary

• Immunogen: E. coli-derived recombinant mouse Cardiotrophin-1
  Ser2-Ala203
  Accession # Q60753
• Specificity: Detects mouse Cardiotrophin-1 in ELISAs and Western blots. In ELISAs, this antibody shows approximately 20% cross-reactivity with recombinant human (rh) Cardiotrophin‑1 and no cross-reactivity with rmIL-11, rmIL-6, rrCNTF, rmLIF, or rmOSM.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cardiotrophin-1/CT-1 Antibody (91907) [Alexa Fluor® 532]

• cardiophin 1
• cardiotrophin 1
• Cardiotrophin1
• Cardiotrophin-1
• CT-1CT1
• CTF1

Background

Cardiotrophin-1 (CT-1) is a member of the cytokine family which also includes IL-6, IL-11, leukemia inhibitory factor (LIF), oncostatin M (OSM), and ciliary neurotrophic factor (CNTF). It was originally isolated based on its ability to induce cardiac myocyte hypertrophy in vitro. CT-1 has since been shown to be a pleiotrophic cytokine with overlapping actions with other IL-6 family members on a variety of cell types. Mouse CT-1 encodes a 203 amino acid (aa) residue protein that lacks a hydrophobic signal peptide. The mechanism of CT-1 release from cells is currently not understood. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity. CT-1 is highly expressed in heart, skeletal muscle, liver, lung and kidney. Lower levels of CT-1 expression is also seen in testis and brain. CT-1 initiates downstream signaling pathways through the heterodimerization of gp130 and the LIF receptor beta subunit. A third alpha receptor subunit has also been implicated in the receptor complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.