CARD6 Antibody

Product Discontinued

CARD6 Antibody Summary

• Immunogen: A synthetic peptide corresponding to amino acids 156-173 (RETALSARKNEKEYDTPE) of human CARD6 was used as immunogen; GenBank no. gi|16554564|ref|NP_115976.2|. The immunogen sequence is 100% conserved in human CINCIN1, a 311 amino acid variant of CARD6; GenBank no. gi|33772099|gb|AAP40637.1|.
• Specificity: CARD6
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CARD6
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Immunoprecipitation 1:50-1:200
  • Western Blot 1:1000-1:2000

Reactivity Notes

Cross reacts with Human.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for CARD6 Antibody

• CARD6
• caspase recruitment domain family, member 6
• caspase recruitment domain protein 6
• caspase recruitment domain-containing protein 6
• CINCIN1
• CINCIN1CARD-containing inhibitor of Nod1 and Cardiak-induced NF-kB activation

Background

CARDs (caspase recruitment domain) are homotypic protein-protein interaction motifs belonging to the death domain-fold (DD) superfamily (reviewed in Werts et al, 2006). Homotypic interactions among CARD-containing proteins can occur with various proteins, including the inactive zymogen forms of caspases which have N-terminal pro-domains containing complementary CARDs. These interactions can result in the formation of multiprotein complexes, leading to protease cleavage and caspase activation. Most proteins containing a CARD are involved in signal transduction pathways that regulate apoptosis, adaptive immunity, or innate immunity including NF-kB activation and inflammation. There is a large number of CARD containing proteins, and the unique function of each remains to be fully elucidated. CARD6 is a CARD-containing protein with a domain structure that doesn't appear to be shared by any other protein (Stehlik et al, 2003). CARD6 has been found to associate with microtubules and is structurally and potentially functionally related to the superfamily of interferon (IFN)-inducible GTPases (Dufner, 2006). IFN-inducible GTPases belong to a growing family of host defense proteins that confer cell immunity. Specifically, is thought that CARD6 may act as a pattern recognition receptor that combats pathogens in a cell-autonomous manner. CARD6 is a 1037 amino acid protein, and CINCIN1 is 311 amino acid splice variants of CARD6. The antibody recognizes both CARD6 and CINCIN1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CARD6
• Entrez:
  • 84674(Human)