Carboxypeptidase A1/CPA1 Antibody (477305)

• Reactivity: Hu
• Applications: WB, IP
• Clone: 477305
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Carboxypeptidase A1/CPA1 Antibody (477305) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A1/CPA1
  Lys17-Tyr419
  Accession # P15085
• Specificity: Detects human Carboxypeptidase A1/CPA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25% cross-reactivity with recombinant mouse (rm) Carboxypeptidase A1 is observed and no cross-reactivity with recombinant human (rh) Carboxypeptidase A2, B1, E, or recombinant mouse Carboxypeptidase A4 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CPA1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carboxypeptidase A1/CPA1 Antibody (477305)

• carboxypeptidase A1 (pancreatic)
• Carboxypeptidase A1
• CPA
• CPA1
• EC 3.4.17
• EC 3.4.17.1

Background

Carboxypeptidase A1 encoded by the CPA1 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic or branched aliphatic side chains including Phe, Tyr, Trp, Leu or Ile. It is important in the degradation of food proteins to produce essential amino acids such as Phe and Trp. The deduced amino acid sequence of human CPA1 consists of a signal peptide (residues 1 to 16), a pro region (residue 17 to 110), and a mature chain (residues 111 to 419). The purified rhCPA1 corresponds to the pro form.

1. Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 812, Academic Press, San Diego.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Carboxypeptidase A1/CPA1 Antibody (MAB2856)(1)

Publication using MAB2856

• M Sándor, FG Thiel, M Schmid, A Demcsák, NC Morales Gr, BC Németh, S Vajda, A Hoerning, M Sahin-Tóth Novel p.G250A Mutation Associated with Chronic Pancreatitis Highlights Misfolding-Prone Region in Carboxypeptidase A1 (CPA1) International Journal of Molecular Sciences, 2022-12-07;23(24):. 2022-12-07 [PMID: 36555104] (Human)

Bioinformatics

• Gene Symbol: CPA1
• Uniprot:
  • P15085()