Carboxypeptidase A1/CPA1 Antibody (477305) [Alexa Fluor® 647]

• Applications: WB, IP
• Clone: 477305
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647

Carboxypeptidase A1/CPA1 Antibody (477305) [Alexa Fluor® 647] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A1/CPA1
  Lys17-Tyr419
  Accession # P15085
• Specificity: Detects human Carboxypeptidase A1/CPA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25% cross-reactivity with recombinant mouse (rm) Carboxypeptidase A1 is observed and no cross-reactivity with recombinant hu
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Carboxypeptidase A1 encoded by the CPA1 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic or branched aliphatic side chains including Phe, Tyr, Trp, Leu or Ile. It is important in the degradation of food proteins to produce essential amino acids such as Phe and Trp. The deduced amino acid sequence of human CPA1 consists of a signal peptide (residues 1 to 16), a pro region (residue 17 to 110), and a mature chain (residues 111 to 419). The purified rhCPA1 corresponds to the pro form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.