Carbonic Anhydrase VA/CA5A Antibody [Alexa Fluor™ Plus 647]

• Reactivity: Hu
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor Plus 647

Carbonic Anhydrase VA/CA5A Antibody [Alexa Fluor™ Plus 647] Summary

• Specificity: Detects human Carbonic Anhydrase VA/CA5A in direct ELISAs and Western blots. In direct ELISAs, approximately 4% cross-reactivity with recombinant human (rh) CA5B is observed and less than 1% cross-reactivity with rhCA2 and rhCA7.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase VA/CA5A Antibody [Alexa Fluor™ Plus 647]

• CA5A
• CA5carbonic anhydrase 5A, mitochondrial
• Carbonate dehydratase VA
• carbonic anhydrase V, mitochondrial
• Carbonic Anhydrase VA
• carbonic anhydrase VA, mitochondrial
• carbonic dehydratase
• CAV
• CAVA
• CA-VA
• EC 4.2.1.1

Background

Carbonic Anhydrase catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1). Topics in a CA meeting (6^th International Conference on the CAs, June 20 - 25, 2003, Slovakia) ranged from the use of CAs as markers for tumor and hypoxia in the clinic, as a nutritional supplement in milk, and as a tool for CO₂ removal and mosquito control in industry. Carbonic anhydrase VA encoded by the CA5A gene is a mitochondrial protein (2, 3). In comparison with another mitochondrial CA (CA5B), CA5A has different tissue distribution and chromosomal location (4, 5). Expression and inhibitor studies of different CAs in the rat pancreatic beta cells indicate that CA5A may be involved in the regulation of insulin secretion (6). CA5A may also participate in the detoxification of ammonia produced in the gastrointestinal tract by providing bicarbonate to carbamyl phosphate synthetase I (7). The amino acid sequence of recombinant human CA5A (residues 40 to 305) is 79%, 77%, and 76% identical to that of canine, bovine, and rat/mouse.

1. Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
2. Nagao, Y. et al. (1993) Proc. Natl. Acad. Sci. USA 90:7623.
3. Nagao, Y. et al. (1995) Genomics 28:477.
4. Shah, G.N. et al. (2000) Proc. Natl. Acad. Sci. USA 97:1677.
5. Fujikawa-Adachi, K. et al. (1999) J. Biol. Chem. 274:21228.
6. Parkkila, A.K. et al. (1998) J. Biol. Chem. 273:24620.
7. Saarnio, J. et al. (1999) J. Histochem. Cytochem. 47:517.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.