Carbonic Anhydrase IV/CA4 Antibody (565112)

Carbonic Anhydrase IV was detected in perfusion fixed frozen sections of mouse small intestine using Mouse Carbonic Anhydrase IV Monoclonal Antibody (Catalog # MAB2414) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to nuclei, cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Carbonic Anhydrase IV was detected in perfusion fixed frozen sections of mouse kidney using Mouse Carbonic Anhydrase IV Monoclonal Antibody (Catalog # MAB2414) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Product Discontinued

Carbonic Anhydrase IV/CA4 Antibody (565112) Summary

• Immunogen:

  Mouse myeloma cell line NS0-derived recombinant mouse Carbonic Anhydrase IV

  
  Glu18-Ser277
  Accession # Q64444
• Specificity: Detects mouse CA4 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CA1, 2, 3, 4, 5A, 5B, 6, 7, 8, 9, 10, 11, 12, 13, 14, recombinant mouse CA9, 12, or 14 is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: CA4
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbonic Anhydrase IV/CA4 Antibody (565112)

• CA4
• CAIV
• CA-IV
• Car4
• Carbonate dehydratase IV
• carbonic anhydrase 4
• Carbonic Anhydrase IV
• carbonic anhydrase IVRP17
• carbonic dehydratase IV
• EC 4.2.1.1
• retinitis pigmentosa 17 (autosomal dominant)
• RP17

Background

Carbonic Anhydrase IV (CA4) is a 32 kDa GPI-anchored membrane zinc metalloenzyme that is involved in bicarbonate transport and pH regulation. CA4 is expressed on the luminal surfaces of renal proximal tubules and pulmonary and retinal capillaries. Loss of function mutation of CA4 function is associated with retinal degeneration. Mature mouse and human CA4 share 55% amino acid sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CA4
• Uniprot:
  • Q64444()