Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein

SDS-Page: Recombinant E. coli Carbonic Anhydrase II/CA2 Protein [NBP2-52240] - 15% SDS Page (3 ug)

• Reactivity: Ec
• Applications: PAGE, Enzyme Activity
• Concentration: 1 mg/ml

Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein Summary

• Description: A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-220 of E.coli Carbonic Anhydrase II/CA2
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MKDIDTLISN NALWSKMLVE EDPGFFEKLA QAQKPRFLWI GCSDSRVPAE RLTGLEPGEL FVHRNVANLV IHTDLNCLSV VQYAVDVLEV EHIIICGHYG CGGVQAAVEN PELGLINNWL LHIRDIWFKH SSLLGEMPQE RRLDTLCELN VMEQVYNLGH STIMQSAWKR GQKVTIHGWA YGIHDGLLRD LDVTATNRET LEQRYRHGIS NLKLKHANHK

• Details of Functionality: Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: CA2
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
  • SDS-Page
• Theoretical MW: 27.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant E. coli Carbonic Anhydrase II/CA2 His Protein

• CA2
• CAII
• CA-IIEC 4.2.1.1
• Car2
• Carbonate dehydratase II
• carbonic anhydrase 2
• carbonic anhydrase B
• Carbonic anhydrase C
• Carbonic Anhydrase II
• carbonic anhydrase IICAC
• carbonic dehydratase

Background

Carbonic anhydrase II (CAII) is a single polypeptide chain of molecular weight 29kDa. It is present in the cytosol of most tissues, but highest concentrations are found, like Carbonic Anhydrase I, in erythrocytes. The concentration in erythrocytes is about 20

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: CA2