Reactivity | HuSpecies Glossary |
Applications | Func, PAGE, Bioactivity |
Concentration | 1 mg/ml |
Description | A bioactive recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-261 of Human Carbonic Anhydrase I/CA1 Source: E.coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MASPDWGYDD KNGPEQWSKL YPIANGNNQS PVDIKTSETK HDTSLKPISV SYNPATAKEI INVGHSFHVN FEDNDNRSVL KGGPFSDSYR LFQFHFHWGS TNEHGSEHTV DGVKYSAELH VAHWNSAKYS SLAEAASKAD GLAVIGVLMK VGEANPKLQK VLDALQAIKT KGKRAPFTNF DPSTLLPSSL DFWTYPGSLT HPPLYESVTW IICKESISVS SEQLAQFRSL LSNVEGDNAV PMQHNNRPTQ PLKGRTVRAS F |
Details of Functionality | Specific activity is > 300 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8.0 at 37C. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | CA1 |
Purity | >95% pure by SDS-PAGE |
Theoretical MW | 31 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer | 20 mM Tris-HCl buffer (pH 8.0), 1 mM DTT, 10% glycerol |
Preservative | No Preservative |
Concentration | 1 mg/ml |
Purity | >95% pure by SDS-PAGE |
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Gene Symbol | CA1 |