Carbohydrate Sulfotransferase 3/CHST3 Antibody (799011) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: IHC
• Clone: 799011
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

Carbohydrate Sulfotransferase 3/CHST3 Antibody (799011) [Alexa Fluor® 750] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human Carbohydrate Sulfotransferase 3/CHST3
  Glu39-Thr479
  Accession # Q7LGC8
• Specificity: Detects human Carbohydrate Sulfotransferase 3/CHST3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human CHST1 or recombinant mouse CHST3 is observed.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Carbohydrate Sulfotransferase 3/CHST3 Antibody (799011) [Alexa Fluor® 750]

• C6ST1
• C6ST-1
• C6ST1HSD
• C6STEC 2.8.2.17
• carbohydrate (chondroitin 6) sulfotransferase 3
• Carbohydrate Sulfotransferase 3
• Chondroitin 6-O-sulfotransferase 1
• Chondroitin 6-sulfotransferase
• CHST3
• EC 2.8.2
• Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0
• GST-0

Background

The human CHST family is comprised of 14 enzymes. All members of this family are Golgi-localized type II membrane proteins. Only the luminal and enzymatic domain is expressed in each of our recombinant CHST proteins. These enzymes transfer sulfate (i.e., sulfonate) onto the 6-O or 4-O positions of GalNAc, Gal and GlcNAc residues on glycoproteins, proteoglycans and glycolipids (1). This sulfation often creates specific epitopes that can be recognized by extracellular matrix proteins, cell surface receptors and viruses (2). CHST3, also known as chondroitin 6-O-sulfotransferase, transfers sulfate to position 6 of GalNAc residues on chondroitin sulfate (3). Chondroitin sulfate constitutes the predominant proteoglycan present in cartilage and is distributed on the surfaces of many cells and extracellular matrices. Loss of CHST3 function in human results in severe chondrodysplasia (4). CHST3 can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides (5). The enzymatic activity of the recombinant human CHST3 was measured using a phosphatase-coupled sulfotransferease assay (6).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.