CAPZA2 Antibody

Western Blot: CAPZA2 Antibody [NBP2-92643] - Analysis of extracts of various cell lines, using CAPZA2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry-Paraffin: CAPZA2 Antibody [NBP2-92643] - Immunohistochemistry of paraffin-embedded Mouse kidney using CAPZA2 Rabbit pAb (NBP2-92643) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry-Paraffin: CAPZA2 Antibody [NBP2-92643] - Immunohistochemistry of paraffin-embedded Human colon using CAPZA2 Rabbit pAb (NBP2-92643) at dilution of 1:50 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

CAPZA2 Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-286 of human CAPZA2 (NP_006127.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAPZA2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS with 50% glycerol, pH7.3.
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for CAPZA2 Antibody

• CAPPA2CAPZ
• capping protein (actin filament) muscle Z-line, alpha 2
• CapZ alpha-2
• F-actin capping protein alpha-2 subunit
• F-actin-capping protein subunit alpha-2

Background

The protein encoded by the CAPZA2 gene is a member of the F-actin capping protein alpha subunit family. It is the alphasubunit of the barbed-end actin binding protein Cap Z. By capping the barbed end of actin filaments, Cap Z regulatesthe growth of the actin filaments at the barbed end. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAPZA2