Cannabinoid R2/CB2/CNR2 Antibody (352110) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with either (A) human Cannabinoid R2/CB2/ CNR2 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human Cannabinoid R2/CB2/CNR2 Monoclonal Antibody (Catalog # MAB36551) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: 352110
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Cannabinoid R2/CB2/CNR2 Antibody (352110) [Unconjugated] Summary

• Immunogen: Human embryonic kidney cell line HEK293-derived transfected with human Cannabinoid R2/CB2/CNR2
  Met1-Cys360
  Accession # NP_001832
• Specificity: Detects human Cannabinoid R2/CB2/CNR2 in direct ELISAs. Stains human Cannabinoid R2/CB2/CNR2 transfectants but not irrelevant transfectants in flow cytometry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cannabinoid R2/CB2/CNR2 Antibody (352110) [Unconjugated]

• Cannabinoid R2
• cannabinoid receptor 2 (macrophage)
• CannabinoidR2
• CB-2
• CB2cannabinoid receptor 2
• CB2R
• CNR2
• CX5
• CX5testis-dominant CNR2 isoform CB2
• hCB2

Background

Cannabinoid R2 (Cannabinoid Receptor 2), also called CB2 or CNR2, is a 7TM protein that belongs to the family of G protein-coupled receptors, class A. Cannabinoid R2 is expressed in the periphery, notably by hematopoietic cells, in contrast to Cannabinoid R1 which is expressed predominantly in the central nervous system and gut. Cannabinoid R2 on lymphocytes and macrophages binds cannabinoids and modulates cytokine secretion. With the exception of their divergent C‑terminal tails, human Cannabinoid R2 shares 82% amino acid sequence identity with mouse and rat Cannabinoid R2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cannabinoid R2/CB2/CNR2 Antibody (MAB36551)(2)

Publications using MAB36551

• A Yeliseev, A van den Be, L Zoubak, K Hines, S Stepnowski, K Williston, W Yan, K Gawrisch, J Zmuda Thermostability of a recombinant G protein-coupled receptor expressed at high level in mammalian cell culture Sci Rep, 2020-10-08;10(1):16805. 2020-10-08 [PMID: 33033368] (Western Blot, E. coli)
• A Bartoll, A Toledano-Z, J Casas, M Guzmán, EH Schuchman, MD Ledesma Inhibition of fatty acid amide hydrolase prevents pathology in neurovisceral acid sphingomyelinase deficiency by rescuing defective endocannabinoid signaling EMBO Mol Med, 2020-10-05;0(0):e11776. 2020-10-05 [PMID: 33016621] (ICC, IHC, Western Blot, Human, Mouse)

Bioinformatics

• Uniprot:
  • NP_001832()