Cannabinoid R1/CB1/CNR1 Antibody

Western Blot: Cannabinoid R1/CB1/CNR1 Antibody [NB100-2427] - Western blot analysis of HeLa lysate non-glycosylated (A) + peptide (B) with antibody at 1 ug/mL and HeLa lysate glycosylated (C) + peptide (D) with antibody at 0.5 ug/mL. 35 ug protein in RIPA buffer. Detected by chemiluminescence.

• Reactivity: Mu, Rt, Hu, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Cannabinoid R1/CB1/CNR1 Antibody Summary

• Description: Novus Biologicals Goat Cannabinoid R1/CB1/CNR1 Antibody (NB100-2427) is a polyclonal antibody validated for use in WB and ELISA. Anti-Cannabinoid R1/CB1/CNR1 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-HTSEDGKVQVTRPDQ corresponding to internal region according to NP_001153732.1, NP_001153731.1, NP_001153730.1, NP_001153698.1, NP_149421.1.
• Specificity: This antibody is expected to recognize both reported isoforms. Isoform a (NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1) and isoform b (NP_149421.1).
• Predicted Species: Human (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CNR1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 2 ug/mL
• Application Notes: Western blot: Approx 55 kDa (non-glycosylated) and 65 kDa (glycosylated) bands observed in lysates of cell line HeLa (calculated MW of 52.9 kDa according to NP_057167.2;). Both bands were successfully blocked by incubation with the immunizing peptide,
• Control:

  Human Brain Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24291518).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Cannabinoid R1/CB1/CNR1 Antibody

• CANN6
• CANN6CB1R
• Cannabinoid R1
• cannabinoid receptor 1 (brain)
• CannabinoidR1
• CB1
• CB1A
• CB1K5
• CB1R
• CB-R
• CB-RCB1cannabinoid receptor 1
• central cannabinoid receptor
• CNR
• CNR1
• SKR6R

Background

Gene ID(s): 1268 (human)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cannabinoid R1/CB1/CNR1 Antibody (NB100-2427)(4)

Publications using NB100-2427

• Perdikaris P, Tsarouchi M, Fanarioti E et al. Long lasting effects of chronic WIN55,212-2 treatment on mesostriatal dopaminergic and cannabinoid systems in the rat brain. Neuropharmacology 2018-02-01 [PMID: 29113897]
• Mouton-Liger F, Sahun I, Collin T et al. Developmental molecular and functional cerebellar alterations induced by PCP4/PEP19 overexpression: Implications for Down syndrome. Neurobiol Dis 2014-03-01 [PMID: 24291518] (WB, Mouse)
• Porcella A, Maxia C, Gessa GL et al. The human eye expresses high levels of CB1 cannabinoid receptor mRNA and protein. Eur J Neurosci 2000-03-01 [PMID: 10762343]
• Ledent C, Valverde O, Cossu G et al. Unresponsiveness to cannabinoids and reduced addictive effects of opiates in CB1 receptor knockout mice. Science 1999-01-15 [PMID: 9888857]

Bioinformatics

• Gene Symbol: CNR1
• Entrez:
  • 1268(Human)
  • 12801(Mouse)
  • 25248(Rat)
• Uniprot:
  • NP_057167.2(Human)