Canine CCL2/MCP-1 Quantikine ELISA Kit

• Reactivity: Ca
• Applications: ELISA
• Conjugate: HRP

Canine CCL2/MCP-1 Quantikine ELISA Kit Summary

• Background: The Quantikine Canine MCP-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure canine MCP-1 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant canine MCP-1 and antibodies raised against the recombinant protein. Results obtained for naturally occurring canine MCP-1 showed linear curves that were parallel to the standard curves obtain...ed using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural canine MCP-1.
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• Specificity: Natural and recombinant canine MCP-1
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: CCL2

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Canine CCL2/MCP-1 Quantikine ELISA Kit

• C-C motif chemokine ligand 2
• CCL2
• GDCF-2
• HC11
• HSMCR30
• MCAF
• Mcp1
• MCP-1
• SCYA2
• SMC-CF

Background

CCL2/JE/MCP-1, is a chemokine that binds the receptor CCR2 and induces the chemoattraction of mononuclear cells. It induces the activation of monocytes, NK cells, lymphocytes, and basophils. Additionally, CCL2 promotes Th2 polarization in CD4+ T cells, and CCL2-mediated recruitment of monocytes to sites of inflammation contributes to disease severity in atherosclerosis, multiple sclerosis, and allergic asthma. Endogenous proteolytic trimming of CCL2 at the N-terminus, including the N-terminal pyrrolidone carboxylic acid-modified glutamine, downregulates activity but not receptor binding.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for CCL2/JE/MCP-1 (CACP00)(5)

Publications using CACP00

• Pantaleo, V;Furlanello, T;Ventura, L;Solano-Gallego, L; Serum and urinary monocyte chemoattractant protein-1 as markers of inflammation and renal damage in dogs with naturally occurring leishmaniosis Parasites & vectors 2024-08-29 [PMID: 39210379] (Canine)
• J Davis, G Rossi, DW Miller, RE Cianciolo, AL Raisis Ability of different assay platforms to measure renal biomarker concentrations during ischaemia-reperfusion acute kidney injury in dogs Res Vet Sci, 2020-11-12;0(0):. 2020-11-12 [PMID: 33223120] (Canine)
• K Hashimoto, Y Kobatake, R Asahina, O Yamato, MS Islam, H Sakai, H Nishida, S Maeda, H Kamishina Up-regulated inflammatory signatures of the spinal cord in canine degenerative myelopathy Res Vet Sci, 2020-11-05;0(0):. 2020-11-05 [PMID: 33187678] (IHC, Canine)
• C Raghu, J Ekena, JM Cullen, CB Webb, LA Trepanier Evaluation of potential serum biomarkers of hepatic fibrosis and necroinflammatory activity in dogs with liver disease J. Vet. Intern. Med., 2018-02-27;0(0):. 2018-02-27 [PMID: 29485210] (Canine)
• Naoaki Matsuki CC chemokine ligand 2 and CXC chemokine ligand 8 as neutrophil chemoattractant factors in canine idiopathic polyarthritis Vet. Immunol. Immunopathol., 2016-09-23;182(0):52-58. 2016-09-23 [PMID: 27863550] (Canine)

FAQs for CCL2/JE/MCP-1 (CACP00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CCL2