CaMKIV Antibody (1A3) - Azide and BSA Free

Western Blot: CaMKIV Antibody (1A3) [H00000814-M01] - Analysis of CAMK4 expression in transfected 293T cell line by CAMK4 monoclonal antibody (M01), clone 1A3.Lane 1: CAMK4 transfected lysate(52.14 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: CaMKIV Antibody (1A3) [H00000814-M01] - Analysis of monoclonal antibody to CAMK4 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: CaMKIV Antibody (1A3) [H00000814-M01] - Analysis of monoclonal antibody to CAMK4 on formalin-fixed paraffin-embedded human tonsil tissue. Antibody concentration 5 ug/ml.

Western Blot: CaMKIV Antibody (1A3) [H00000814-M01] - Analysis of CAMK4 over-expressed 293 cell line, cotransfected with CAMK4 Validated Chimera RNAi ( Cat # H00000814-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK4 monoclonal antibody (M01) clone 1A3 (Cat # H00000814-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Western Blot: CaMKIV Antibody (1A3) [H00000814-M01] - CAMK4 monoclonal antibody (M01), clone 1A3 Analysis of CAMK4 expression in Jurkat.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF, IHC, KD
• Clone: 1A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

CaMKIV Antibody (1A3) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: CAMK4 (AAH25687.1, 1 a.a. ~ 473 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MLKVTVPSCSASSCSSVTASAAPGTASLVPDYWIDGSNRDALSDFFEVESELGRGATSIVYRCKQKGTQKPYALKVLKKTVDKKIVRTEIGVLLRLSHPNIIKLKEIFETPTEISLVLELVTGGELFDRIVEKGYYSERDAADAVKQILEAVAYLHENGIVHRDLKPENLLYATPAPDAPLKIADFGLSKIVEHQVLMKTVCGTPGYCAPEILRGCAYGPEVDMWSVGIITYILLCGFEPFYDERGDQFMFRRILNCEYYFISPWWDEVSLNAKDLVRKLIVLDPKKRLTTFQALQHPWVTGKAANFVHMDTAQKKLQEFNARRKLKAAVKAVVASSRLGSASSSHGSIQESHKASRDPSPIQDGNEDMKAIPEGEKIQGDGAQAAVKGAQAELMKVQALEKVKGADINAEEAPKMVPKAVEDGIKVADLELEEGLAEEKLKTVEEAAAPREGQGSSAVGFEVPQQDVILPEY
• Specificity: CAMK4 - calcium/calmodulin-dependent protein kinase IV
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CAMK4
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Knockdown Validated
  • Western Blot 1:500
• Application Notes: Antibody reactivity against recominant protein and cell lysate for WB. It has been used for IF, IHC-P, RNAi Validation and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for CaMKIV Antibody (1A3) - Azide and BSA Free

• brain Ca(2+)-calmodulin-dependent protein kinase type IV
• brain Ca++-calmodulin-dependent protein kinase type IV
• calcium/calmodulin-dependent protein kinase IV
• calcium/calmodulin-dependent protein kinase type IV catalytic chain
• calcium/calmodulin-dependent protein kinase type IV
• CAM kinase- GR
• CAM kinase IV
• CaM kinase-GR
• CaMK IV
• caMK
• CaMK-GR
• EC 2.7.11
• EC 2.7.11.17
• IV
• MGC36771

Background

The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CaMKIV Antibody (H00000814-M01)(1)

Publication using H00000814-M01

• Nakanishi M, Hata K, Nagayama T et al. Acid activation of Trpv1 leads to an up-regulation of calcitonin gene-related peptide expression in dorsal root ganglion neurons via the CaMK-CREB cascade: a potential mechanism of inflammatory pain. Mol Biol Cell. 2010-06-09 [PMID: 20534813]

Bioinformatics

• Gene Symbol: CAMK4
• Entrez:
  • 814(Human)
• OMIM:
  • 114080(Human)
• Uniprot:
  • AAH25687(Human)