CaM Kinase II alpha [p Thr286] Antibody

Images

 
Western Blot: CaMKII alpha [p Thr286] Antibody [NB300-184] - Anaylsis of Rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k beta-CaM Kinase II phosphorylated at Thr286 (Control). The ...read more

Product Details

Summary
Reactivity Rt, Human, Mouse, XenopusSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit

Order Details

CaM Kinase II alpha [p Thr286] Antibody Summary

Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr286 found in rat brain CaM Kinase II.
Modification
p Thr286
Localization
Cell membrane; presynaptic membrane. Cell junction; synapse.
Specificity
Specific for the ~50k alpha-CaM Kinase II and the ~60k beta-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the gamma-phosphatase treatment
Predicted Species
Human (100%), Mouse (100%), Xenopus (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
CAMK2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Publications
Read Publications using NB300-184.

Alternate Names for CaM Kinase II alpha [p Thr286] Antibody

  • calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
  • calcium/calmodulin-dependent protein kinase II alpha
  • calcium/calmodulin-dependent protein kinase II alpha-B subunit
  • CaM kinase II alpha subunit
  • CaM Kinase II alpha
  • CaM kinase II subunit alpha
  • CAMK2A
  • CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
  • CaMK-II alpha subunit
  • CaMK-II subunit alpha
  • CaMKIINalpha
  • CaM-kinase II alpha chain
  • EC 2.7.11
  • EC 2.7.11.17
  • KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Background

CaM KII is an important member of calcium/calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling. CaM KII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaM KII further autophosphorylates itself at Thr286 to render the kinase constitutively active. The threonine phosphorylation state of CaM KII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaM KII at Thr286. On the other hand, PKA (protein kinase A) prevents this phosphorylation process through its inhibitory effect on PP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-13006
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP2-14871
Species: Hu, Mu
Applications: WB, ICC/IF
NBP1-98474
Species: Hu
Applications: WB
NB120-2860
Species: Hu, Mu, Rt, Ba, Bv, Ch
Applications: WB, ELISA, Flow, IHC, IHC-P, ICC, IF
AF2180
Species: Hu
Applications: WB, Simple Western, IP
NBP1-47842
Species: Hu, Rt, Ca, Mk
Applications: WB, IHC, IHC-P, IP
NB100-82245
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NB100-410
Species: Hu, Mu
Applications: WB, IP
NB110-75039
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
NB300-106
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IP
NB100-858
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-Fr, PEP-ELISA, IHC-WhMt
MAB18251
Species: Hu, Mu
Applications: WB, Simple Western
PPS007
Species: Hu, Mu, Rt, Ca, Ch
Applications: WB
NB300-556
Species: Hu, Mu, Rt, In, Pm
Applications: WB, ChIP, B/N, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB120-22131
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P

Publications for CaM Kinase II alpha Antibody (NB300-184)(5)

Reviews for CaM Kinase II alpha Antibody (NB300-184) (0)

There are no reviews for CaM Kinase II alpha Antibody (NB300-184). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CaM Kinase II alpha Antibody (NB300-184) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional CaM Kinase II alpha [p Thr286] Antibody Products

Related Products by Gene

Bioinformatics Tool for CaM Kinase II alpha Antibody (NB300-184)

Discover related pathways, diseases and genes to CaM Kinase II alpha Antibody (NB300-184). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CaM Kinase II alpha Antibody (NB300-184)

Discover more about diseases related to CaM Kinase II alpha Antibody (NB300-184).
 

Pathways for CaM Kinase II alpha Antibody (NB300-184)

View related products by pathway.

PTMs for CaM Kinase II alpha Antibody (NB300-184)

Learn more about PTMs related to CaM Kinase II alpha Antibody (NB300-184).
 

Research Areas for CaM Kinase II alpha Antibody (NB300-184)

Find related products by research area.

Blogs on CaM Kinase II alpha

There are no specific blogs for CaM Kinase II alpha, but you can read our latest blog posts.

Contact Information

Product PDFs

Bioinformatics

Gene Symbol CAMK2A
Entrez
Uniprot

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NB300-184 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought