CaM Kinase II alpha [p Thr286] Antibody

Images

 
Western Blot: CaMKII alpha [p Thr286] Antibody [NB300-184] - Anaylsis of Rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k beta-CaM Kinase II phosphorylated at Thr286 (Control). The ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, ZeSpecies Glossary
Applications WB, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CaM Kinase II alpha [p Thr286] Antibody Summary

Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr286 conjugated to KLH
Modification
p Thr286
Localization
Cell membrane; presynaptic membrane. Cell junction; synapse.
Specificity
Specific for the ~50k alpha-CaM Kinase II and the ~60k beta-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the gamma-phosphatase treatment
Predicted Species
Zebrafish (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAMK2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry-Paraffin
Application Notes
Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 28972541).
Theoretical MW
50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
CaM Kinase II alpha Lysate (NBL1-08661)
Reviewed Applications
Read 7 Reviews rated 4.9
using
NB300-184 in the following applications:

Publications
Read Publications using
NB300-184 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 28972541).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for CaM Kinase II alpha [p Thr286] Antibody

  • calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
  • calcium/calmodulin-dependent protein kinase II alpha
  • calcium/calmodulin-dependent protein kinase II alpha-B subunit
  • CaM kinase II alpha subunit
  • CaM Kinase II alpha
  • CaM kinase II subunit alpha
  • CAMK2A
  • CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
  • CaMK-II alpha subunit
  • CaMK-II subunit alpha
  • CaMKIINalpha
  • CaM-kinase II alpha chain
  • EC 2.7.11
  • EC 2.7.11.17
  • KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Background

CaM KII is an important member of calcium/calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling. CaM KII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaM KII further autophosphorylates itself at Thr286 to render the kinase constitutively active. The threonine phosphorylation state of CaM KII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaM KII at Thr286. On the other hand, PKA (protein kinase A) prevents this phosphorylation process through its inhibitory effect on PP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CaM Kinase II alpha Antibody (NB300-184)(6)

Reviews for CaM Kinase II alpha Antibody (NB300-184) (7) 4.97

Average Rating: 4.9
(Based on 7 reviews)
We have 7 reviews tested in 4 species: Human, Mouse, Human and Mouse, Mouse and Human.

Reviews using NB300-184:
Filter by Applications
WB
(4)
ICC
(1)
Flow
(2)
All Applications
Filter by Species
Human
(2)
Mouse
(3)
Human and Mouse
(1)
Mouse and Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 06/13/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman macrophage
SpeciesHuman
Lotks715p
 
reviewed by:
Anonymous
ICC Mouse 06/13/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedAorta tissue
SpeciesMouse
Lotks715p
 
reviewed by:
Bishuang Cai
Flow Mouse 06/13/2017
View

Summary

ApplicationFlow Cytometry
Sample TestedPeritoneal macrophages
SpeciesMouse
Lotks715p
 
reviewed by:
Bishuang Cai
WB Mouse 06/13/2017
View

Summary

ApplicationWestern Blot
Sample TestedBone Marrrow Derived Macrophages
SpeciesMouse
Lotks715p
 
reviewed by:
Bishuang Cai
Flow Human 05/24/2017
View

Summary

ApplicationFlow Cytometry
Sample Testedhuman macrophage
SpeciesHuman
Lotks715p
 
reviewed by:
Bishuang Cai
WB Mouse and Human 05/02/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman macrophage,Bone Marrrow Derived Macrophages
SpeciesMouse and Human
Lotks715p
 
reviewed by:
Anonymous
WB Human and Mouse 04/17/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse macrophages,human macrophage
SpeciesHuman and Mouse
Lotks715p

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Control Lysate(s)

Secondary Antibodies

 

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Recent Reviews

4.9
5
6
4
1
3
0
2
0
1
0

 
Anonymous
06/13/2017
Application: WB
Species: Human

 
Anonymous
06/13/2017
Application: ICC
Species: Mouse

 
Bishuang Cai
06/13/2017
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol CAMK2A
Entrez
Uniprot