CaM Kinase II alpha [p Thr286] Antibody

Images

 
Western Blot: CaMKII alpha [p Thr286] Antibody [NB300-184] - Anaylsis of Rat brain lysate showing specific immunolabeling of the ~50k a- and the ~60k beta-CaM Kinase II phosphorylated at Thr286 (Control). The ...read more

Product Details

Summary
Reactivity Mu, Rt, Hu, ZeSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

CaM Kinase II alpha [p Thr286] Antibody Summary

Immunogen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Thr286 found in rat brain CaM Kinase II.
Modification
p Thr286
Localization
Cell membrane; presynaptic membrane. Cell junction; synapse.
Specificity
Specific for the ~50k alpha-CaM Kinase II and the ~60k beta-CaM Kinase II proteins phosphorylated at Thr286. Immunolabeling is blocked by the gamma-phosphatase treatment
Predicted Species
Human (100%), Zebrafish (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
CAMK2A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Positive Control
CaM Kinase II alpha Lysate (NBL1-08661)
Reviewed Applications
Read 7 Reviews rated 4.9
using
NB300-184 in the following application:

Publications
Read Publications using NB300-184.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for CaM Kinase II alpha [p Thr286] Antibody

  • calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
  • calcium/calmodulin-dependent protein kinase II alpha
  • calcium/calmodulin-dependent protein kinase II alpha-B subunit
  • CaM kinase II alpha subunit
  • CaM Kinase II alpha
  • CaM kinase II subunit alpha
  • CAMK2A
  • CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
  • CaMK-II alpha subunit
  • CaMK-II subunit alpha
  • CaMKIINalpha
  • CaM-kinase II alpha chain
  • EC 2.7.11
  • EC 2.7.11.17
  • KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Background

CaM KII is an important member of calcium/calmodulin-activated kinase, functioning in neural synaptic stimulation and T-cell receptor signaling. CaM KII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaM KII further autophosphorylates itself at Thr286 to render the kinase constitutively active. The threonine phosphorylation state of CaM KII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaM KII at Thr286. On the other hand, PKA (protein kinase A) prevents this phosphorylation process through its inhibitory effect on PP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-14871
Species: Hu, Mu
Applications: WB, ICC/IF
NBP1-98474
Species: Hu
Applications: WB
NB120-2860
Species: Hu, Mu, Rt, Ba, Bv, Ch
Applications: WB, ELISA, Flow, IHC, IHC-P, ICC, IF
AF2180
Species: Hu
Applications: WB, Simple Western, IP
NBP2-25162
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NB100-410
Species: Hu, Mu
Applications: WB, IP
NBP2-50037
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF
NB300-106
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-Fr, IP
NB100-858
Species: Hu, Mu, Rt, GP, Mk
Applications: WB, ICC/IF, IHC, IHC-Fr, PEP-ELISA, IHC-WhMt
NBP2-22203
Species: Hu, Mu, Rt, Mk
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
NB300-171
Species: Mu, Rt
Applications: WB, IHC, IHC-Fr
NB300-556
Species: Hu, Mu, Rt, In, Pm
Applications: WB, ChIP, B/N, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB120-22131
Species: Hu, Mu, Rt
Applications: WB, ICC/IF
NB100-56708
Species: Hu, Mu, Rt, Po, ChHa, Ma
Applications: WB, Simple Western, EM, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, CyTOF-ready

Publications for CaM Kinase II alpha Antibody (NB300-184)(5)

Reviews for CaM Kinase II alpha Antibody (NB300-184) (7) 4.97

Average Rating: 4.9
(Based on 7 reviews)
We have 7 reviews tested in 4 species: Human, Mouse, Human and Mouse, Mouse and Human.
We have 7 reviews tested in 3 application: WB, ICC, Flow.

Reviews using NB300-184:
Filter by Applications
WB
(4)
ICC
(1)
Flow
(2)
All Applications
Filter by Species
Human
(2)
Mouse
(3)
Human and Mouse
(1)
Mouse and Human
(1)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
WB Human 06/13/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman macrophage
SpeciesHuman
Lotks715p
 
reviewed by:
Anonymous
ICC Mouse 06/13/2017
View

Summary

ApplicationImmunocytochemistry
Sample TestedAorta tissue
SpeciesMouse
Lotks715p
 
reviewed by:
Bishuang Cai
Flow Mouse 06/13/2017
View

Summary

ApplicationFlow Cytometry
Sample TestedPeritoneal macrophages
SpeciesMouse
Lotks715p
 
reviewed by:
Bishuang Cai
WB Mouse 06/13/2017
View

Summary

ApplicationWestern Blot
Sample TestedBone Marrrow Derived Macrophages
SpeciesMouse
Lotks715p
 
reviewed by:
Bishuang Cai
Flow Human 05/24/2017
View

Summary

ApplicationFlow Cytometry
Sample Testedhuman macrophage
SpeciesHuman
Lotks715p
 
reviewed by:
Bishuang Cai
WB Mouse and Human 05/02/2017
View

Summary

ApplicationWestern Blot
Sample Testedhuman macrophage,Bone Marrrow Derived Macrophages
SpeciesMouse and Human
Lotks715p
 
reviewed by:
Anonymous
WB Human and Mouse 04/17/2017
View

Summary

ApplicationWestern Blot
Sample TestedMouse macrophages,human macrophage
SpeciesHuman and Mouse
Lotks715p

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for CaM Kinase II alpha Antibody (NB300-184) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional CaM Kinase II alpha [p Thr286] Antibody Products

Related Products by Gene

Bioinformatics Tool for CaM Kinase II alpha Antibody (NB300-184)

Discover related pathways, diseases and genes to CaM Kinase II alpha Antibody (NB300-184). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for CaM Kinase II alpha Antibody (NB300-184)

Discover more about diseases related to CaM Kinase II alpha Antibody (NB300-184).
 

Pathways for CaM Kinase II alpha Antibody (NB300-184)

View related products by pathway.

PTMs for CaM Kinase II alpha Antibody (NB300-184)

Learn more about PTMs related to CaM Kinase II alpha Antibody (NB300-184).
 

Research Areas for CaM Kinase II alpha Antibody (NB300-184)

Find related products by research area.

Blogs on CaM Kinase II alpha

There are no specific blogs for CaM Kinase II alpha, but you can read our latest blog posts.

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4.9
5
6
4
1
3
0
2
0
1
0

 
Anonymous
06/13/2017
Application: WB
Species: Human

 
Anonymous
06/13/2017
Application: ICC
Species: Mouse

 
Bishuang Cai
06/13/2017
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol CAMK2A
Entrez
Uniprot

Customers Who Bought This Also Bought