CaM Kinase II alpha Antibody - BSA Free

Western Blot: CaM Kinase II alpha Antibody [NB100-81830] - Analysis of extracts from Hela using NB100-81830

Western Blot: CaM Kinase II alpha Antibody [NB100-81830] - Western blotting analysis using NB100-81830

Immunohistochemistry-Paraffin: CaM Kinase II alpha Antibody - BSA Free [NB100-81830] - Immunofluorescence staining of paraffin-embedded human hippocampal region tissue from the falling sickness disease using NB100-81830.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CaM Kinase II alpha Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit CaM Kinase II alpha Antibody - BSA Free (NB100-81830) is a polyclonal antibody validated for use in IHC and WB. Anti-CaM Kinase II alpha Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human CaM Kinase II alpha around amino acids 284~288 (Q-E-T-V-D).
• Specificity: Detects endogenous levels of total CaMKII protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAMK2A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500-1:1000
• Application Notes: Use in Immunohistochemistry-paraffin reported in scientific literature (PMID: 28912554).
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  CaM Kinase II alpha Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CaM Kinase II alpha Antibody - BSA Free

• calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
• calcium/calmodulin-dependent protein kinase II alpha
• calcium/calmodulin-dependent protein kinase II alpha-B subunit
• CaM kinase II alpha subunit
• CaM Kinase II alpha
• CaM kinase II subunit alpha
• CAMK2A
• CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
• CaMK-II alpha subunit
• CaMK-II subunit alpha
• CaMKIINalpha
• CaM-kinase II alpha chain
• EC 2.7.11
• EC 2.7.11.17
• KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Background

CaMKII (Calcium/calmodulin-dependent protein kinase II) is a ubiquitous serine/threonine protein kinase that is abundant in the brain as a major constituent of the postsynaptic density (PSD). The enzyme is an oligomeric protein composed of distinct but related subunits, alpha, beta, gamma, and delta, each encoded by a separate gene. CAMK2A assembles into heterooligomeric complexes with other CAMK2 subunits. CaMKII is a prominent kinase in the central nervous system that may function in long term potentiation and neurotransmitter release.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CaM Kinase II alpha Antibody (NB100-81830)(4)

Publications using NB100-81830

• Tsuneki H, Sugiyama M, Ito T et al. Food odor perception promotes systemic lipid utilization Nature metabolism 2022-11-01 [PMID: 36376564] (IHC-Fr, Mouse)
  
  Details:
  Dilution used in IHC-Fr 1:100
• Khoo GH, Lin YT, Tsai TC, Hsu KS. et Al. Perineuronal Nets Restrict the Induction of Long-Term Depression in the Mouse Hippocampal CA1 Region Mol Neurobiol 2019-03-04 [PMID: 30826967]
• Yu-Ting Lin, Tsan-Yu Hsieh, Tsung-Chih Tsai, Chien-Chung Chen, Chiung-Chun Huang, Kuei-Sen Hsu Conditional Deletion of Hippocampal CA2/CA3a Oxytocin Receptors Impairs the Persistence of Long-Term Social Recognition Memory in Mice The Journal of Neuroscience 2018-01-31 [PMID: 29279308]
• Lin YT, Chen CC, Huang CC, Nishimori K Oxytocin stimulates hippocampal neurogenesis via oxytocin receptor expressed in CA3 pyramidal neurons Nat Commun. 2017-09-13 [PMID: 28912554] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: CAMK2A
• Entrez:
  • 815(Human)
  • 12322(Mouse)
  • 25400(Rat)
• Uniprot:
  • Q9UQM7()