CaM Kinase II alpha [p Thr286] Antibody (SR1853) - BSA Free

Western Blot: CaM Kinase II alpha [p Thr 286] Antibody (SR1853) [NBP3-22608] - Western blot analysis using NBP3-22608 in Mouse brain treated with Lambda phosphatase cell lysate.

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clone: SR1853
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

CaM Kinase II alpha [p Thr286] Antibody (SR1853) - BSA Free Summary

• Description: Novus Biologicals Rabbit CaM Kinase II alpha [p Thr286] Antibody (SR1853) - BSA Free (NBP3-22608) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-CaM Kinase II alpha [Thr286] (Uniprot #: Q9UQM7, P11798, P11275)
• Modification: p Thr286
• Specificity: Detects endogenous levels of total phospho-CaM Kinase II alpha [Thr286]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: CAMK2A
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for CaM Kinase II alpha [p Thr286] Antibody (SR1853) - BSA Free

• calcium/calmodulin-dependent protein kinase (CaM kinase) II alpha
• calcium/calmodulin-dependent protein kinase II alpha
• calcium/calmodulin-dependent protein kinase II alpha-B subunit
• CaM kinase II alpha subunit
• CaM Kinase II alpha
• CaM kinase II subunit alpha
• CAMK2A
• CAMKAcalcium/calmodulin-dependent protein kinase type II subunit alpha
• CaMK-II alpha subunit
• CaMK-II subunit alpha
• CaMKIINalpha
• CaM-kinase II alpha chain
• EC 2.7.11
• EC 2.7.11.17
• KIAA0968calcium/calmodulin-dependent protein kinase type II alpha chain

Background

Calcium/calmodulin-dependent protein kinase II (CaMKII) is a widely distributed protein kinase that regulates numerous physiological functions. Inhibitors of CaMKII are useful tools for investigating the CaMKII functions (1). The synapse contains densely localized and interacting proteins that enable it to adapt to changing inputs. A Ca2+-sensitive protein complex involved in the regulation of AMPA receptor synaptic plasticity. The complex is comprised of MUPPI, a multi-PDZ domain-containing protein; SynGAP, a synaptic GTPase-activating protein; and the Ca2+/calmodulin-dependent kinase CaMKII. In synapses of hippocampal neurons, SynGAP and CaMKII are brought together by direct physical interaction with the PDZ domains of MUPP1, and in this complex, SynGAP is phosphorylated. Ca2+CaM binding to CaMKII dissociates it from the MUPP1 complex, and Ca2+ entering via the NMDAR drives the dephosphorylation of SynGAP (2).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CAMK2A