CaM Kinase I Antibody [Alexa Fluor® 488]

• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

CaM Kinase I Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human CaM Kinase I alpha
  Ser176-Arg254 (Ala224Ser)
  Accession # Q14012
• Specificity: Detects recombinant human CaM Kinase I alpha in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CaMKIV is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

CaMKI (Calcium/Calmodulin-dependent kinase I) is a 37-43 kDa monomeric and cytosolic member of the CaMK subfamily, Ser/Thr protein kinase family, protein kinase superfamily of molecules.  It is found in multiple cell types, including adrenal cortical cells, neurons and macrophages/osteoclast precursors.  CaMKI is associated with the cellular Ca⁺⁺ signaling pathway.  Upon entry into the cell, calcium levels are sensed by calmodulin.  Upon Ca⁺⁺ binding, calmodulin (and CaMK kinase) interacts with a Ser/Thr protein kinase termed CaMKIa.  CaMKIa is of particular interest in neurons and cells of the zona glomerulosa.  In neurons, CaMKIa activation results in the recruitment of AMPA-Rs and the promotion of axon growth (vs.dendrite outgrowth which is promoted by CaMKIg). In the adrenal gland, CaMKIa regulates CYP11B2 transcription, and thus the ability to synthesize aldosterone.  Human CaMKI, the isoform used for immunization, is 370 amino acids (aa) in length (SwissProt #:Q14012).  It contains one protein kinase domain (aa 20-276) followed by a calmodulin-binding region (aa 296-317) and an NES (aa 315-321).   Over aa 178-257, human and mouse CaMKI are identical in amino acid sequence.  There are three additional CaMKI isoforms (b, g, d), all the product of distinct genes.  Nevertheless, over the same aa sequence, these three isoforms (b, g, d) share 80%, 81% and 97% aa sequence identity with CaMKI(a), respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.