Calretinin Antibody

Western Blot: Calretinin Antibody [NBP2-50029] - Western blot analysis of different tissue lysates using chicken pAb to calretinin, NBP2-50029, dilution 1:1,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. A band at 29kDa corresponds to calretinin protein. The same blot was simultaneously probed with mouse mAb to alpha-nternexin, dilution 1:10,000 in red that reveals the alpha-internexin protein with apparent molecular weight of 66kDa.

Immunohistochemistry Free-Floating: Calretinin Antibody [NBP2-50029] - Analysis of rat hippocampus section stained with chicken pAb to calretinin, NBP2-50029, dilution 1:1,000 in green, and costained with mouse mAb to parvalbumin, 1:1,000 in red. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with the above antibodies. The calretinin and parvalbumin antibodies label different classes of interneurons.

• Reactivity: Hu, Mu, Rt, Po, Bv, Eq
• Applications: WB, ICC/IF, IHC, IHC-FrFl
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated

Calretinin Antibody Summary

• Immunogen: Recombinant full length human Calretinin. [UniProt# P22676]
• Specificity: This antibody does not cross-react with the related calcium binding proteins Calbindin D-28K and Parvalbumin.
• Isotype: IgY
• Clonality: Polyclonal
• Host: Chicken
• Gene: CALB2
• Purity: Ammonium sulfate precipitation

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000 - 1:5000
  • Immunocytochemistry/Immunofluorescence 1:1000 - 1:5000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry Free-Floating
• Application Notes: In Western blot, this antibody recognizes a clear band at ~29 kDa in rat brain lysates and it reacts only with Calretinin protein, and not other calcium-binding proteins.
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 5mM Sodium Azide
• Purity: Ammonium sulfate precipitation

Alternate Names for Calretinin Antibody

• 29 kDa calbindin
• CAB29
• CAL2
• CALB2
• calbindin 2
• calbindin 2, (29kD, calretinin)
• calbindin 2, 29kDa (calretinin)
• calbindin D29K
• Calretinin
• CR

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALB2
• Uniprot:
  • P22676(Human)