Calretinin Antibody (7H1G3) - BSA Free

Western Blot: Calretinin Antibody (7H1G3) [NBP2-61720] - Analysis using CALB2 mAb against HEK293 (1) and CALB2 (AA: 172-271)-hIgGFc transfected HEK293 (2) cell lysate.

Flow Cytometry: Calretinin Antibody (7H1G3) [NBP2-61720] - Analysis of Hela cells using CALB2 mouse mAb (green) and negative control (red).

Western Blot: Calretinin Antibody (7H1G3) [NBP2-61720] - Analysis using CALB2 mAb against human CALB2 (AA: 172-271) recombinant protein. (Expected MW is 39.2 kDa)

Western Blot: Calretinin Antibody (7H1G3) [NBP2-61720] - Analysis using CALB2 mouse mAb against HepG2 (1) and COS7 (2) cell lysate.

ELISA: Calretinin Antibody (7H1G3) [NBP2-61720] - Black line: Control Antigen (100 ng);Purple line: Antigen (10ng); Blue line: Antigen (50 ng); Red line:Antigen (100 ng)

Product Discontinued

Calretinin Antibody (7H1G3) - BSA Free Summary

• Immunogen: Purified recombinant fragment of human CALB2 (AA: 172-271) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: CALB2
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Western Blot 1:500-1:2000
• Theoretical MW: 31.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for Calretinin Antibody (7H1G3) - BSA Free

• 29 kDa calbindin
• CAB29
• CAL2
• CALB2
• calbindin 2
• calbindin 2, (29kD, calretinin)
• calbindin 2, 29kDa (calretinin)
• calbindin D29K
• Calretinin
• CR

Background

This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Alternative splicing results in multiple transcript variants. ;

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CALB2