![Western Blot: Calretinin Antibody (1F5H1) [NBP2-52426] - Analysis using CALB2 mAb against human CALB2 (AA: 1-271) recombinant protein. (Expected MW is 57.5 kDa)](http://images.novusbio.com/fullsize2/Calretinin-Antibody-(1F5H1)-Western-Blot-NBP2-52426-img0002.jpg "Western Blot: Calretinin Antibody (1F5H1) [NBP2-52426] - Analysis using CALB2 mAb against human CALB2 (AA: 1-271) recombinant protein. (Expected MW is 57.5 kDa)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, ELISA, Flow, IHC, IHC-P, CyTOF-ready |
| Clone | 1F5H1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Immunogen | Purified recombinant fragment of human CALB2 (AA: 1-271) expressed in E. Coli. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | CALB2 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | This antibody is Cytof ready. |
| Theoretical MW | 31.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein A or G purified |
Secondary Antibodies |
Isotype Controls |
Diseases for Calretinin Antibody (NBP2-52426)Discover more about diseases related to Calretinin Antibody (NBP2-52426).
| Pathways for Calretinin Antibody (NBP2-52426)View related products by pathway.
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Using RPE65 as a tool to investigate ocular gene therapies While not life threatening, blindness and retinal disease are profoundly debilitating and greatly affect quality of life. Understandably, gene therapy has been subject to controversy given it’s potential effects on the rest of our cellular... Read full blog post. |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | CALB2 |
![Immunohistochemistry-Paraffin: Calretinin Antibody (1F5H1) [NBP2-52426] - Immunohistochemical analysis of paraffin-embedded brain tissues using CALB2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/Calretinin-Antibody-(1F5H1)-Immunohistochemistry-Paraffin-NBP2-52426-img0004.jpg "Immunohistochemistry-Paraffin: Calretinin Antibody (1F5H1) [NBP2-52426] - Immunohistochemical analysis of paraffin-embedded brain tissues using CALB2 mouse mAb with DAB staining.")
![Flow Cytometry: Calretinin Antibody (1F5H1) [NBP2-52426] - Analysis of A549 cells using Calretinin mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize2/Calretinin-Antibody-(1F5H1)-Flow-Cytometry-NBP2-52426-img0005.jpg "Flow Cytometry: Calretinin Antibody (1F5H1) [NBP2-52426] - Analysis of A549 cells using Calretinin mouse mAb (green) and negative control (red).")
![Western Blot: Calretinin Antibody (1F5H1) [NBP2-52426] - Analysis using CALB2 mAb against HEK293 (1) and CALB2 (AA: 1-271)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/Calretinin Antibody (1F5H1)-Western Blot-NBP2-52426-img0003.jpg "Western Blot: Calretinin Antibody (1F5H1) [NBP2-52426] - Analysis using CALB2 mAb against HEK293 (1) and CALB2 (AA: 1-271)-hIgGFc transfected HEK293 (2) cell lysate.")
![ELISA: Calretinin Antibody (1F5H1) [NBP2-52426] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).](http://images.novusbio.com/fullsize2/Calretinin-Antibody-(1F5H1)-ELISA-NBP2-52426-img0006.jpg "ELISA: Calretinin Antibody (1F5H1) [NBP2-52426] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).")
![Immunocytochemistry/Immunofluorescence: Histatin 3 Antibody (4G9) [H00003347-M01] - Analysis of monoclonal antibody to HTN3 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize2/Histatin-3-Antibody-(4G9)-Immunocytochemistry-Immunofluorescence-H00003347-M01-img0005.jpg)
![Sandwich ELISA: Histatin 3 Antibody (4G9) [H00003347-M01] - Detection limit for recombinant GST tagged HTN3 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/Histatin 3 Antibody (4G9)-Sandwich ELISA-H00003347-M01-img0006.jpg)
![Western Blot: NOD2 Antibody (2D9) [NB100-524] - Total protein from human THP1 and US-OS cells and mouse Raw264.7 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-NOD2 in 1% non-fat milk in TBST and detected with an anti-mouse HRP secondary antibody using chemiluminescence.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Western-Blot-NB100-524-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: NOD2 Antibody (2D9) [NB100-524] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-NOD2 (2D9) NB100-524 at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was counterstained with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunocytochemistry-Immunofluorescence-NB100-524-img0008.jpg)
![Immunohistochemistry-Frozen: NOD2 Antibody (2D9) [NB100-524] - Overlay of NOD2-DyLight 488 (green) with phase contrast of murine colon. Image from verified customer review.](http://images.novusbio.com/fullsize2/NOD2-Antibody-(2D9)-Immunohistochemistry-Frozen-NB100-524-img0016.jpg)
![Flow Cytometry: HLA DQ Antibody (SPV-L3) [NBP2-45041] - Flow Cytometric Analysis of Raji cells. HLA DQ Antibody (SPV-L3) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize2/HLA DQ Antibody (SPV-L3)-Flow Cytometry-NBP2-45041-img0001.jpg)
![Western Blot: Calbindin D-28K Antibody [NBP2-50028] - Analysis of different tissue lysates and recombinant protein solutions using chicken pAb to calbindin, NBP2-50028, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cerebellum, [3] pig hippocampus, [4] cow cerebellum, [5] protein standard (red). Next lanes are full length recombinant human proteins, [6] Parvalbumin, [7] Calretinin, [8] Calbindin. Bands at 25kDa in tissue lysates and ~30 kDa in protein solutions correspond to calbindin, the recombinant form being slightly larger due to the presence of a His tag and other vector derived sequence. NBP2-50028 antibody specifically recognizes calbindin protein and does not react with the closely related proteins parvalbumin and calretinin.](http://images.novusbio.com/fullsize2/Calbindin-D-28K-Antibody-Western-Blot-NBP2-50028-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Calbindin D-28K Antibody [NBP2-50028] - Cultured mouse embryonic kidney stained with Calbindin antibody (1:200) and DAPI. Image from verified customer review.](http://images.novusbio.com/fullsize2/Calbindin-D-28K-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-50028-img0005.jpg)
![Immunohistochemistry Free-Floating: Calbindin D-28K Antibody [NBP2-50028] - Analysis of rat cerebellum section stained with chicken Calbindin D-28K pAb, dilution 1:2,000 (Green), and costained with rabbit MeCP2 pAb, dilution 1:5,000 (Red). Following transcardial perfusion with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. Calbindin D-28K, often used as a Purkinje cell marker, is prominently detected in dendrites and perikarya of these cells in the cerebellar molecular layer. The MeCP2 antibody selectively stains nuclei of neuronal cells.](http://images.novusbio.com/fullsize2/Calbindin-D-28K-Antibody-Immunohistochemistry-Free-Floating-NBP2-50028-img0006.jpg)
![Western Blot: Parvalbumin Antibody [NB120-11427] - WB analysis of rat cerebellum extract.](http://images.novusbio.com/fullsize2/Parvalbumin-Antibody-Western-Blot-NB120-11427-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Parvalbumin Antibody [NB120-11427] - Analysis of Parvalbumin using anti-Parvalbumin polyclonal antibody shows staining in U251 Cells. Parvalbumin staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Parvalbumin at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize2/Parvalbumin-Antibody-Immunocytochemistry-Immunofluorescence-NB120-11427-img0008.jpg)
![Immunohistochemistry-Paraffin: Parvalbumin Antibody [NB120-11427] - Normal biopsies of deparaffinized human tonsil tissue.](http://images.novusbio.com/fullsize2/Parvalbumin-Antibody-Immunohistochemistry-Paraffin-NB120-11427-img0005.jpg)
![Western Blot: PMEL17/SILV Antibody [NBP1-69571] - Sample Tissue: Human MCF7 Antibody Dilution: 1.0 ug/ml](http://images.novusbio.com/fullsize2/PMEL17-SILV-Antibody-Western-Blot-NBP1-69571-img0006.jpg)
![Immunohistochemistry: PMEL17/SILV Antibody [NBP1-69571] - Human kidney](http://images.novusbio.com/fullsize2/PMEL17-SILV-Antibody-Immunohistochemistry-NBP1-69571-img0005.jpg)
![Western Blot: PMEL17/SILV Antibody [NBP1-69571] - This Anti-SILV antibody was used in Western Blot of HepG2 tissue lysate at a concentration of 1.25ug/ml.](http://images.novusbio.com/fullsize2/PMEL17-SILV-Antibody-Western-Blot-NBP1-69571-img0003.jpg)
![Western Blot: Chromogranin C Antibody [NBP2-15895] - A. 50 ug mouse brain lysate/extract 7.5% SDS-PAGE Chromogranin C antibody dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Chromogranin-C-Antibody-Western-Blot-NBP2-15895-img0009.jpg)
![Immunohistochemistry-Paraffin: Chromogranin C Antibody [NBP2-15895] - Paraffin-embedded mouse fore brain. Chromogranin C antibody dilution: 1:500.](http://images.novusbio.com/fullsize2/Chromogranin-C-Antibody-Immunohistochemistry-Paraffin-NBP2-15895-img0007.jpg)
![Western Blot: Chromogranin C Antibody [NBP2-15895] - Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/Chromogranin C Antibody-Western Blot-NBP2-15895-img0001.jpg)
![Immunohistochemistry-Frozen: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Adult mouse small intestine. NB600-534 Siglec-1 was used at 1:500 and labeled with Alexa Fluor 568 conjugated secondary antibody (Red). DAPI shown as blue. Image from verified customer review.](http://images.novusbio.com/fullsize2/Siglec-1-CD169-Antibody-(HSn-7D2)-Immunohistochemistry-Frozen-NB600-534-img0003.jpg)
![Flow Cytometry: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Human CD14+ PBMC differentiated to M1 macrophages with rhGM-CSF were stained with Mouse Anti-Siglec-1/CD169 Monoclonal Antibody (NB600-534, filled histogram), or Mouse IgG1 isotype control (MAB002, open histogram) followed by APC-conjugated Anti-Mouse IgG Secondary Antibody (F0101B).](http://images.novusbio.com/fullsize2/Siglec-1-CD169-Antibody-(HSn-7D2)-Flow-Cytometry-NB600-534-img0002.jpg)
![Immunohistochemistry-Paraffin: Siglec-1/CD169 Antibody (HSn 7D2) [NB600-534] - Staining of Sialoadhesin in human spleen using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize2/Sialoadhesin-Antibody-(HSn-7D2)-Immunohistochemistry-Paraffin-NB600-534-img0001.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunohistochemistry-Paraffin: SI Sucrase-Isomaltase Antibody [NBP1-87581] - Staining of human stomach shows no positivity in glandular cells as expected.](http://images.novusbio.com/fullsize2/SI-Sucrase-Isomaltase-Antibody-Immunohistochemistry-Paraffin-NBP1-87581-img0005.jpg)
![Immunohistochemistry-Paraffin: SI Sucrase-Isomaltase Antibody [NBP1-87581] - Analysis in human small intestine and stomach tissues. Corresponding SI RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/SI-Sucrase-Isomaltase-Antibody-Immunohistochemistry-Paraffin-NBP1-87581-img0001.jpg)
![Immunohistochemistry-Paraffin: SI Sucrase-Isomaltase Antibody [NBP1-87581] - Staining of human small intestine shows moderate to strong membranous positivity in glandular cells.](http://images.novusbio.com/fullsize2/SI-Sucrase-Isomaltase-Antibody-Immunohistochemistry-Paraffin-NBP1-87581-img0002.jpg)
![Western Blot: GRB2 Antibody [NB100-866] - (0.05ug/ml) staining of Mouse (A+C) and Rat (B+D) Brain (A+B) and Spleen (C+D) lysates (35ug total protein in RIPA buffer). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.](http://images.novusbio.com/fullsize2/GRB2-Antibody-Western-Blot-NB100-866-img0012.jpg)
![Immunohistochemistry-Paraffin: GRB2 Antibody [NB100-866] - Negative Control showing staining of paraffin embedded Human Lymph Node, with no primary antibody.](http://images.novusbio.com/fullsize2/GRB2-Antibody-Immunohistochemistry-Paraffin-NB100-866-img0014.jpg)
![Western Blot: GRB2 Antibody [NB100-866] - (0.03ug/ml) staining of Human (A) and Pig (B) Spleen lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize2/GRB2-Antibody-Western-Blot-NB100-866-img0010.jpg)
![Western Blot: ARSH Antibody [NBP1-59370] - Titration: 0.2-1 ug/ml, Positive Control: MCF7 cell lysate.](http://images.novusbio.com/fullsize2/ARSH Antibody-Western Blot-NBP1-59370-img0003.jpg)
![Western Blot: RB1 Antibody [NB100-56888] - Analysis of Retinoblastoma 1 in 30 ugs of Jurkat cell lysate using NB100-56888 at 1:1000 dilution.](http://images.novusbio.com/fullsize2/RB1-Antibody-Western-Blot-NB100-56888-img0003.jpg)
![Western Blot: Catalase Antibody [NBP2-24916] - Analysis in liver lysate of 1) human 2) mouse and 3) rat at 0.1 ug/ml.](http://images.novusbio.com/fullsize2/Catalase-Antibody-Western-Blot-NBP2-24916-img0001.jpg)
![Immunohistochemistry-Paraffin: Catalase Antibody [NBP2-24916] - Staining of human liver tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.](http://images.novusbio.com/fullsize2/Catalase-Antibody-Immunohistochemistry-Paraffin-NBP2-24916-img0002.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Insulin E2E3, human pancreas (FFPE), HIER pH 9 antigen retrieval. Image from verified customer review.](http://images.novusbio.com/fullsize2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0004.jpg)
![Immunohistochemistry-Paraffin: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Formalin-fixed, paraffin-embedded human pancreas stained with insulin Monoclonal Antibody (E2-E3)](http://images.novusbio.com/fullsize2/Insulin-Antibody-(E2-E3-(same-as-INS04))-Immunohistochemistry-Paraffin-NBP2-34260-img0002.jpg)
![Western Blot: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of ITGA2 on A431 cells lysates using anti-ITGA2 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize2/Integrin alpha 2-CD49b Antibody (SN0752)-Western Blot-NBP2-67691-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Staining ITGA2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Integrin-alpha-2-CD49b-Antibody-(SN0752)-Immunocytochemistry-Immunofluorescence-NBP2-67691-img0003.jpg)
![Immunohistochemistry-Paraffin: Integrin alpha 2/CD49b Antibody (SN0752) [NBP2-67691] - Analysis of paraffin-embedded mouse stomach tissue using anti-ITGA2 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Integrin-alpha-2-CD49b-Antibody-(SN0752)-Immunohistochemistry-Paraffin-NBP2-67691-img0008.jpg)
![Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.](http://images.novusbio.com/fullsize2/ER-alpha-NR3A1-Antibody-(33)-Western-Blot-NB300-560-img0004.jpg)
![Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Paraffin-NB300-560-img0010.jpg)
![Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).](http://images.novusbio.com/fullsize2/ER-alpha-NR3A1-Antibody-(33)-Immunohistochemistry-Frozen-NB300-560-img0009.jpg)
![SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](http://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")