Calponin 1 Antibody (CNN1/832 + CALP) - IHC-Prediluted

Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/832 + CALP) - IHC-Prediluted [NBP2-48206] - Formalin-fixed, paraffin-embedded Rat Uterus stained with Calponin-1 Mouse Monoclonal Antibody (CNN1/832 + CALP).

Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/832 + CALP) - IHC-Prediluted [NBP2-48206] - Formalin-fixed, paraffin-embedded human Uterus stained with Calponin-1 Mouse Monoclonal Antibody (CNN1/832 + CALP).

Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/832 + CALP) - IHC-Prediluted [NBP2-48206] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Calponin-1 Mouse Monoclonal Antibody (CNN1/832 + CALP).

• Reactivity: Hu, Rt
• Applications: IHC, IHC-P
• Clone: CNN1/832 + CALP
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: IHC-Prediluted

Calponin 1 Antibody (CNN1/832 + CALP) - IHC-Prediluted Summary

• Immunogen: Recombinant full-length human CNN1 protein (CNN1/832); Crude human uterus extract (CALP)
• Localization: Cytoplasmic
• Marker: Smooth Muscle Marker
• Specificity: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
• Isotype: IgG1 Kappa/IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CNN1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
• Application Notes: Hu-chromosome location: 1q32.2
  Molecular weight of antigen: 90-110kDa
  Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 34 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Notes

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Alternate Names for Calponin 1 Antibody (CNN1/832 + CALP) - IHC-Prediluted

• Basic calponin
• Calponin 1
• calponin 1, basic, smooth muscle
• Calponin H1
• Calponin H1, smooth muscle
• calponins, basic
• CNN1
• Sm-Calp
• SMCC
• SMCCcalponin-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CNN1