Calponin 1 Antibody (CNN1/832 + CALP) - IHC-Prediluted

Images

 
Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/832 + CALP) - IHC-Prediluted [NBP2-48206] - Formalin-fixed, paraffin-embedded Rat Uterus stained with Calponin-1 Mouse Monoclonal Antibody (CNN1/832 + CALP).
Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/832 + CALP) - IHC-Prediluted [NBP2-48206] - Formalin-fixed, paraffin-embedded human Uterus stained with Calponin-1 Mouse Monoclonal Antibody (CNN1/832 + CALP).
Immunohistochemistry-Paraffin: Calponin 1 Antibody (CNN1/832 + CALP) - IHC-Prediluted [NBP2-48206] - Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Calponin-1 Mouse Monoclonal Antibody (CNN1/832 + ...read more

Product Details

Summary
Reactivity Hu, RtSpecies Glossary
Applications IHC, IHC-P
Clone
CNN1/832 + CALP
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
IHC-Prediluted

Order Details

Calponin 1 Antibody (CNN1/832 + CALP) - IHC-Prediluted Summary

Immunogen
Recombinant full-length human CNN1 protein (CNN1/832); Crude human uterus extract (CALP)
Localization
Cytoplasmic
Marker
Smooth Muscle Marker
Specificity
Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist; a 34kDa form and a 29kDa form. Expression of the 29kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
Isotype
IgG1 Kappa/IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
CNN1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
Hu-chromosome location: 1q32.2
Molecular weight of antigen: 90-110kDa
Immunohistochemistry-Paraffin 0.25 - 0.5 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

The prediluted antibody does not require any mixing, dilution, reconstitution, or titration; the antibody is ready-to-use and optimized for staining.

Alternate Names for Calponin 1 Antibody (CNN1/832 + CALP) - IHC-Prediluted

  • Basic calponin
  • Calponin 1
  • calponin 1, basic, smooth muscle
  • Calponin H1
  • Calponin H1, smooth muscle
  • calponins, basic
  • CNN1
  • Sm-Calp
  • SMCC
  • SMCCcalponin-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-47758AF350
Alexa Fluor 405 NBP2-47758AF405
Alexa Fluor 488 NBP2-47758AF488
Alexa Fluor 532 NBP2-47758AF532
Alexa Fluor 594 NBP2-47758AF594
Alexa Fluor 647 NBP2-47758AF647
Alexa Fluor 700 NBP2-47758AF700
Alexa Fluor 750 NBP2-47758AF750
Allophycocyanin NBP2-47758APC
Biotin NBP2-47758B
DyLight 350 NBP2-47758UV
DyLight 405 NBP2-47758V
DyLight 488 NBP2-47758G
DyLight 550 NBP2-47758R
DyLight 594 NBP2-47758DL594
DyLight 650 NBP2-47758C
DyLight 680 NBP2-47758FR
DyLight 755 NBP2-47758IR
FITC NBP2-47758F
FITC NBP2-44482F
HRP NBP2-47758H
Janelia Fluor 549 NBP2-47758JF549
Janelia Fluor 646 NBP2-47758JF646
PE NBP2-47758PE
PerCP NBP2-47758PCP

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Bioinformatics

Gene Symbol CNN1