Calpain S1 Antibody

Western Blot: Calpain S1 Antibody [NBP2-15677] - Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE gel, diluted at 1:1000.

Immunohistochemistry-Paraffin: Calpain S1 Antibody [NBP2-15677] - Immunohistochemical analysis of paraffin-embedded LUNG ADC, using antibody at 1:100 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

Calpain S1 Antibody Summary

• Description: Novus Biologicals Rabbit Calpain S1 Antibody (NBP2-15677) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Calpain S1. The exact sequence is proprietary.
• Localization: Cytoplasm; Cell membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAPNS1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 28 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Calpain S1 Antibody

• Calcium-activated neutral proteinase small subunit
• Calcium-dependent protease small subunit 1
• calcium-dependent protease, small subunit
• calpain 4, small subunit (30K)
• Calpain regulatory subunit
• calpain small subunit 1
• calpain, small polypeptide
• CALPAIN4
• CANP
• CANPS
• CAPN4
• CDPS
• CSS1
• epididymis secretory sperm binding protein

Background

This gene is a member of the calpain small subunit family. Calpains are calcium-dependent cysteine proteinases that are widely distributed in mammalian cells. Calpains operate as heterodimers, comprising a specific large catalytic subunit (calpain 1 subunit in Calpain I, and calpain 2 subunit in Calpain II), and a common small regulatory subunit encoded by this gene. This encoded protein is essential for the stability and function of both calpain heterodimers, whose proteolytic activities influence various cellular functions including apoptosis, proliferation, migration, adhesion, and autophagy. Calpains have been implicated in neurodegenerative processes, such as myotonic dystrophy. A pseudogene of this gene has been defined on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CAPNS1
• Uniprot:
  • P04632(Human)