Calpain 1 Antibody - BSA Free

Western Blot: Calpain 1 Antibody [NBP2-15670] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: Calpain 1 Antibody [NBP2-15670] - Confocal immunofluorescence analysis of methanol-fixed A431, using Calpain 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.

Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15670] - Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.

Western Blot: Calpain 1 Antibody [NBP2-15670] - Sample (30 ug of whole cell lysate) A: whole zebrafish 7.5% SDS PAGE NBP2-15670 diluted at 1:1000

Immunohistochemistry: Calpain 1 Antibody [NBP2-15670] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Calpain 1 antibody (NBP2-15670) at 1:300 dilution.

• Reactivity: Hu, Ze
• Applications: WB, Flow, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Calpain 1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit Calpain 1 Antibody - BSA Free (NBP2-15670) is a polyclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.
• Localization: Cytoplasm; Cell membrane
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CAPN1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 82 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Chicken (80%), Rat (87%), Xenopus laevis (86%), Xenopus tropicalis (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris, 0.1M Glycine, 20% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for Calpain 1 Antibody - BSA Free

• Calcium-activated neutral proteinase 1
• Calpain 1
• calpain 1, (mu/I) large subunit
• Calpain mu-type
• calpain, large polypeptide L1
• calpain-1 catalytic subunit
• Calpain-1 large subunit
• CANP 1
• CANP
• CANPL1
• CANPL1EC 3.4.22.52
• CAPN1
• Cell proliferation-inducing gene 30 protein
• cell proliferation-inducing protein 30
• EC 3.4.22
• EC 3.4.22.52
• Micromolar-calpain
• muCANP
• muCANPCANP1
• muCL
• PIG30

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: CAPN1
• Uniprot:
  • P07384(Human)