Calpain 1 Antibody

Images

 
Western Blot: Calpain 1 Antibody [NBP2-15670] - Sample (30 ug of whole cell lysate) A: Jurkat 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Calpain 1 Antibody [NBP2-15670] - Confocal immunofluorescence analysis of methanol-fixed A431, using Calpain 1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are ...read more
Immunohistochemistry-Paraffin: Calpain 1 Antibody [NBP2-15670] - Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Concentration
0.55 mg/ml

Order Details

Calpain 1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Calpain 1. The exact sequence is proprietary.
Localization
Cytoplasm; Cell membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
CAPN1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Conjugate
Unconjugated
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.55 mg/ml
Purity
Immunogen affinity purified

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunocytochemistry/Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:10 - 1:500
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
82 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (87%), Chicken (80%), Rat (87%), Xenopus laevis (86%), Xenopus tropicalis (84%).

Alternate Names for Calpain 1 Antibody

  • Calcium-activated neutral proteinase 1
  • calpain 1, (mu/I) large subunit
  • Calpain mu-type
  • calpain, large polypeptide L1
  • calpain-1 catalytic subunit
  • Calpain-1 large subunit
  • CANP 1
  • CANP
  • CANPL1EC 3.4.22.52
  • Cell proliferation-inducing gene 30 protein
  • cell proliferation-inducing protein 30
  • EC 3.4.22
  • micromolar-calpain
  • muCANPCANP1
  • muCL

Background

The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Calpain 1 Antibody (NBP2-15670) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Calpain 1 Antibody (NBP2-15670) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Calpain 1

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol CAPN1
Uniprot

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